Nanosensors and related technologies

• US 9,102,521 B2
• Filed: 06/11/2007
• Issued: 08/11/2015
• Est. Priority Date: 06/12/2006
• Status: Expired due to Fees

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Lieber, Charles M., Fang, Ying, Patolsky, Fernando
• Primary Examiner(s): CROW, ROBERT THOMAS
• Application Number:
  US12/308,207
• Publication Number:
  US 20100087013A1
• Time in Patent Office: 2,983 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2563/155 Particles of a defined size, e.g. nanoparticles
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers
• CPC Class Codes: G01N 27/4145 specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
• CPC Class Codes: G01N 27/4146 involving nanosized elements, e.g. nanotubes, nanowires
• CPC Class Codes: G01N 33/551 the carrier being inorganic
• CPC Class Codes: G01N 33/553 Metal or metal coated
• CPC Class Codes: H10K 85/225 comprising substituents
• CPC Class Codes: H10K 85/761 Biomolecules or bio-macromolecules, e.g. proteins, chlorophyl, lipids or enzymes

Abstract

The present invention generally relates to nanoscale wire devices and methods for use in determining nucleic acids or other analytes suspected to be present in a sample. For example, a nanoscale wire device can be used to detect single base mismatches within a nucleic acid (e.g., by determining association and/or dissociation rates). In one aspect, dynamical information such as a binding constant, an association rate, and/or a dissociation rate, can be determined between an analyte and a binding partner immobilized relative to a nanoscale wire. In some cases, the nanoscale wire includes a first portion comprising a metal-semiconductor compound, and a second portion that does not include a metal-semiconductor compound. The binding partner, in some embodiments, is immobilized relative to at least the second portion of the nanoscale wire, and the size of the second portion of the nanoscale wire may be minimized and/or controlled in some instances.

Claims

1. An article, comprising:

a nanoscale wire comprising a first portion and a second portion laterally defined along the length of the nanoscale wire, the first portion consisting essentially of a single crystal of a transition metal silicide compound, the compound being stoichiometric, and the second portion comprising a semiconductor, having a length of less than 100 nm, and having immobilized relative thereto a binding partner of an analyte, wherein the first portion is free of the binding partner.

2. The article of claim 1, wherein the transition metal comprises nickel.

3. The article of claim 1, wherein the nanoscale wire is part of a FET.

4. The article of claim 1, wherein the nanoscale wire further comprises a third portion being defined laterally along the nanowire, the second portion separating the first portion and the third portion, the third portion consisting essentially of a single crystal of the transition metal-semiconductor compound.

5. The article of claim 1, wherein the second portion has a length of less than 50 nm.

6. The article of claim 1, wherein the second portion has a length of less than 30 nm.

7. The article of claim 1, wherein the second portion further comprises a binding partner to an analyte immobilized relative thereto.

8. The article of claim 7, wherein the analyte is a nucleic acid.

9. The article of claim 7, wherein the binding partner is a nucleic acid.

10. The article of claim 7, wherein the article comprises a plurality of nanoscale wires, each comprising distinguishable nucleic acids immobilized relative thereto.

11. An article, comprising:

a sample exposure region able to hold a solution containing an analyte;

a nanoscale wire positioned at least partially adjacent to or within the sample exposure region, the nanoscale wire comprising a first portion and a second portion, each laterally defined along the nanoscale wire, the second portion comprising a semiconductor and having immobilized relative thereto a binding partner able to bind to the analyte contained in the solution, and the first portion being free of the binding partner and comprising a conductive transition metal silicide compound, the compound being stoichiometric,

wherein the binding partner is able to bind one or more molecules of the analyte, wherein the analyte, when bound to the binding partner, has a Debye screening length within the solution that is greater than the greatest dimension of the second portion of the nanoscale wire.

12. The article of claim 11, wherein the analyte comprises a nucleic acid.

13. The article of claim 11, wherein a single molecule of the analyte, when bound to the binding partner, has a Debye screening length than the greatest dimension of the second portion of the nanoscale wire.

14. The article of claim 11, wherein the transition metal silicide compound consists essentially of a single crystal.

15. The article of claim 11, wherein the nanoscale wire comprises the first portion, the second portion, and a third portion each laterally defined along the nanoscale wire, wherein the third portion is free of the binding partner and comprises a conductive transition metal-semiconductor compound.