Method, apparatus, and system for examining optically a sample carried in a plurality of wells

• US 9,103,932 B2
• Filed: 09/30/2011
• Issued: 08/11/2015
• Est. Priority Date: 10/01/2010
• Status: Active Grant

• Current Assignee: Kemira Oyj
• Original Assignee: Aqsens Oy
• Inventors: Trnkvist, Niklas, Hnninen, Pekka
• Primary Examiner(s): Porta, David
• Assistant Examiner(s): Malevic, Djura
• Application Number:
  US13/877,266
• Publication Number:
  US 20130277545A1
• Time in Patent Office: 1,411 Days
• Field of Search: 250/461.1, 250/462.1, 250/459.1
• US Class Current: 1/1
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6454 using an integrated detector array
• CPC Class Codes: G01N 2201/0626 Use of several LED's for spatial resolution
• CPC Class Codes: G01N 2201/129 Using chemometrical methods
• CPC Class Codes: G01V 8/20 using multiple transmitters or receivers

Abstract

An apparatus and method are disclosed for examining optically a sample carried in a plurality of wells. A holder is adapted to receive and hold in place a sample carrier. A plurality of excitation means selectively introduce excitation towards a spatially limited portion of a sample carrier held in place by said holder. Detecting means receive and detect emission radiation coupled out from a light output window of a sample carrier held in place by said holder. Said detecting means is common to said excitation means and is configured to receive emission radiation from a plurality of different spatially limited portions of a sample carrier held in place by said holder.

Claims

1. A method for examining optically a sample, carried in a plurality of wells, the method comprising:

receiving a sample carrier in a holder and holding said sample carrier in place;

selectively introducing excitation from an excitation means subgroup, where the excitation means subgroup comprises at least two excitation means, towards at least two of the wells of the sample carrier, and wherein each of the two wells comprising;

differently coated or modified surfaces each forming a differently behaving non-specific interacting surface;

a luminophore label;

the sample, and wherein said surfaces are configured to;

interact with the sample but not with the luminophore label;

interact with the luminophore label but not with the sample; and

/or

interact with a combination of the sample and the luminophore label; and

receiving and detecting emission radiation coupled out from the at least two of the wells of the sample carrier;

wherein said receiving and detecting is performed commonly to the excitation means subgroup by receiving and detecting emission radiation with a common detector from a plurality of different spatially limited portions of the sample carrier.

2. The method according to claim 1, wherein the step of selectively introducing excitation comprises switching on a predetermined subgroup of available radiating excitation means for radiating excitation radiation.

3. The method according to claim 2, wherein a number of predetermined subgroups of the available radiating excitation means are switched on in sequence, each subgroup comprising a number of radiating excitation means that is at least one and at most as many as there are available radiating excitation means.

4. The method according to claim 2, wherein said excitation is introduced in pulses, and said emission radiation is detected during a time interval in a range of 100 μ

s to 1000 μ

s following an excitation pulse.

5. The method according to claim 2, wherein at least one of wavelength, intensity, polarization, or exposure of at least one excitation means of the excitation means subgroup is adjusted independently from at least one other excitation means of said excitation means subgroup.

6. The method according to claim 5, further comprising:

executing a sequence of excitation pulses, each excitation pulse implementing a characteristic spatially limited pattern of excitation,

receiving and detecting emission radiation as a response to each excitation pulse, and

storing information that relates the executed sequence of excitation pulses to the received and detected emission radiation as a fingerprint of a sample held by a particular sample carrier.

7. The method according to claim 1, wherein at least one of wavelength, intensity, polarization, or exposure of at least one excitation means of the excitation means subgroup is adjusted independently from at least one other excitation means of said excitation means subgroup.

8. The method according to claim 1, further comprising:

executing a sequence of excitation pulses, each excitation pulse implementing a characteristic spatially limited pattern of excitation,

receiving and detecting emission radiation as a response to each excitation pulse, and

storing information that relates the executed sequence of excitation pulses to the received and detected emission radiation as a fingerprint of a sample held by a particular sample carrier.

9. A method according to claim 1, wherein said excitation is introduced in pulses, and said emission radiation is detected during a time interval in a range of 100 μ

s to 1000 μ

s following an excitation pulse.

10. An apparatus for examining optically a sample, carried in a plurality of wells, the apparatus comprising:

a holder adapted to receive and hold in place a sample carrier;

an excitation means subgroup adapted to selectively introduce excitation towards at least two of the wells of the sample carrier held in place by the holder, where the excitation means subgroup comprises at least two excitation means, and wherein each of the two wells comprising;

differently coated or modified surfaces each forming a differently behaving non-specific interacting surface;

a luminophore label;

the sample, and wherein said surfaces are configured to;

interact with the sample but not with the luminophore label;

interact with the luminophore label but not with the sample; and

/or

interact with a combination of the sample and the luminophore label; and

detecting means for receiving and detecting emission radiation coupled out from the at least two of the wells of said sample carrier held in place by said holder;

wherein the detecting means is common to the excitation means subgroup and is configured to receive emission radiation from a plurality of different spatially limited portions of the sample carrier held in place by the holder.

11. The apparatus according to claim 10, wherein said excitation means comprise radiating excitation means that comprise a regular array of individually controllable radiation sources configured to correspond with a regular array of sample spots or wells in a sample carrier held in place by said holder.

12. The apparatus according to claim 11, wherein the radiating excitation means comprise at least one of the following:

light-emitting diodes, laser sources, output ends of optical fibers.

13. The apparatus according to claim 10, further comprising an excitation controller configured to switch on the excitation means in subgroups, each subgroup comprising a number of excitation means that is at least one and at most as many as there are excitation means in the apparatus.

14. The apparatus according to claim 10, wherein the excitation means have a wavelength, and a wavelength of at least one excitation means differs from the wavelength of another excitation means.

15. The apparatus according to claim 10, wherein at least one excitation means has a controllable wavelength.

16. The apparatus according to claim 10, wherein the excitation means and the detecting means are stationary.

17. The apparatus according to claim 10, wherein the detecting means comprise an emission radiation input window configured to match an emission radiation output window defined by a sample carrier held in place by said holder.

18. The apparatus according to claim 10, wherein at least one of wavelength, intensity, polarization, or exposure of at least one excitation means of the excitation means subgroup is configured to be adjustable independently from at least one other excitation means of said excitation means subgroup.

19. The apparatus according to claim 10, further comprising an identification reader configured to read an identifier of a sample carrier held in place by said holder.

20. A computer program product comprising machine-readable instructions that, when executed by a processor, are configured to cause the processor to implement a method, the method comprising:

as a response to an indication of a sample carrier being held in place by a holder, selectively introducing excitation from an excitation means subgroup towards at least two of the wells of the sample carrier, where said excitation means subgroup comprises at least two excitation means, and wherein each of the two wells comprising;

differently coated or modified surfaces each forming a differently behaving non-specific interacting surface;

a luminophore label;

the sample, and wherein said surfaces are configured to;

interact with the sample but not with the luminophore label;

interact with the luminophore label but not with the sample; and

/or

interact with a combination of the sample and the luminophore label; and

receiving and detecting emission radiation coupled out from said at least two of the wells of said sample carrier;

wherein said receiving and detecting is performed commonly to said excitation means subgroup by receiving and detecting emission radiation with a common detector from a plurality of different spatially limited portions of said sample carrier.

21. An apparatus for optically detecting a fingerprint of luminescent intensities of a sample, the apparatus comprising:

a holder adapted to receive and hold in place a sample carrier, the sample carrier having the sample carried in a plurality of wells;

an excitation means subgroup adapted to selectively introduce excitation towards at least a first and second well of the plurality of wells of the sample carrier held in place by the holder, wherein the excitation means subgroup comprises at least a first and second excitation means, the first excitation means subgroup adapted to radiate excitation radiation at a first wavelength to at least a portion of the first well of the sample carrier and the second excitation means subgroup adapted to radiate excitation radiation at a second wavelength to at least a portion of the second well of the sample carrier, and wherein each of the at least first and second wells comprising;

differently coated or modified surfaces each forming a differently behaving non-specific interacting surface;

a luminophore label; and

the sample;

wherein said differently coated or modified surfaces are configured to;

interact with the sample but not with the luminophore label;

interact with the luminophore label but not with the sample; and

/or

interact with a combination of the sample and the luminophore label; and

detecting means for receiving and detecting emission radiation coupled out from the at least first and second wells of the plurality of wells of the sample carrier held in place by the holder;

wherein the detecting means is common to the excitation means subgroup and is configured to receive emission radiation from a plurality of different spatially limited portions of the sample carrier held in place by the holder.