Robust, rapid, secure sample manipulation before during and after ionization for a spectroscopy system

• US 9,105,435 B1
• Filed: 10/31/2014
• Issued: 08/11/2015
• Est. Priority Date: 04/18/2011
• Status: Active Grant

• Current Assignee: Bruker Scientific LLC (Bruker Corporation)
• Original Assignee: IonSense, Inc. (Bruker Corporation)
• Inventors: Musselman, Brian D
• Primary Examiner(s): Maskell, Michael
• Application Number:
  US14/530,621
• Time in Patent Office: 284 Days
• Field of Search: 250/288
• US Class Current: 1/1
• CPC Class Codes: G01N 2560/00 Chemical aspects of mass spectrometric analysis of biological material
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: H01J 27/022 Details
• CPC Class Codes: H01J 27/24 using photo-ionisation, e.g. using laser beam
• CPC Class Codes: H01J 27/26 using surface ionisation, e.g. field effect ion sources, thermionic ion sources H01J27/20, H01J27/24 take precedence
• CPC Class Codes: H01J 49/10 Ion sources; Ion guns
• CPC Class Codes: H01J 49/14 using particle bombardment, e.g. ionisation chambers

Abstract

This invention provides for the efficient positioning of a sample to be analyzed by using either magnetic or electro-mechanical fields to retain the sample in the ionization region. In an embodiment of the present invention, the sample is contacted with a sampler device, which is inserted into a chamber and accurately positioned using electro-mechanical devices. In an embodiment of the invention, the influence of an electro-mechanical field on the sampler device enables the sample to be positioned in the ionization region to be contacted by particles that result in ionization of the sample whereby rendering the resulting ions available for analysis.

Claims

1. A device for ionizing an analyte comprising:

a chamber with at least one port to allow the analyte to enter the chamber and adapted to mix the analyte with a material;

a lid adapted to close and form a seal with the chamber;

a magnetic field source adapted to constrain the analyte mixed with the material within the sealed chamber; and

an ionization source directed at the analyte mixed with the material to form analyte ions.

2. The device of claim 1, where the magnetic field source is generated by one or both a magnet and an electro-magnet.

3. The device of claim 1, further comprising controlling the position of the sample in the chamber with one or both the presence and absence of one or both of an electric field and the magnetic field source.

4. The device of claim 1, where the position of the sample can be adjusted relative to the ionization source prior to an ionization event.

5. The device of claim 1, where the position of the sample can be adjusted relative to the ionization source during an ionization event.

6. The device of claim 1, where the position of the sample can be adjusted relative to the ionization source after an ionization event.

7. The device of claim 1, where the ionization source is an atmospheric pressure ionization source.

8. The device of claim 7, where the atmospheric pressure ionization is selected from the group consisting of a Direct Ionization in Real Time source, a desorption electro spray ionization (DESI) source, an atmospheric laser desorption ionization source, a Corona discharge source, an inductively coupled plasma (ICP) source and a glow discharge source.

9. The device of claim 1, further comprising a spectroscopic analyzer adapted to analyze the sample ions.

10. The device of claim 9, where the spectroscopic analyzer is selected from the group consisting of a mass spectrometer, Raman spectrometer, electro-magnetic absorption spectrometer, electro-magnetic emission spectrometer, surface detection spectrometer, differential scanning mobility spectrometer and ion mobility mass spectrometer.

11. The device of claim 1, where the material includes a metal with one or both a magnetic dipole moment and an inducible magnetic dipole moment.

12. The device of claim 1, where the sample is selected from the group consisting of a powder, lyophilized sample, plant material, fine grains, and a loose substance.

13. The device of claim 1, where the material and the sample are physically mixed.

14. A method of disposing of an analyzed sample comprising the steps of:

receiving a sample mixed with a material that is heavier than the sample;

sealing the sample in an analysis chamber adapted to close and form a seal such that no additional sample mixed with the material enters the analysis chamber;

analyzing the sample in the analysis chamber the analyzing comprising directing an ionization source at the sample mixed with the material to form sample ions;

determining the analysis of the sample in the analysis chamber is complete; and

removing the sample mixed with the material using a magnetic field to eject the sample from the analysis chamber.

15. The method of claim 14, where the magnetic field is generated by one or both a magnet and an electro-magnet.

16. The method of claim 14, further comprising providing an electric field to position the sample mixed with the material prior to removing the sample.

17. The method of claim 16, further comprising using the magnetic field and the electric field to remove the sample.

18. A method of disposing of an analyzed sample comprising the steps of:

receiving a sample with a material that is heavier than the sample;

inserting the sample with the material in an analysis chamber;

sealing the sample with the material in the analysis chamber adapted to close and form a seal such that no additional sample mixed with the material can enter the analysis chamber;

analyzing the sample in the sealed analysis chamber the analyzing comprising directing an ionization source at the sample mixed with the material to form sample ions;

determining the analysis of the sample in the sealed analysis chamber is complete; and

removing the sample mixed with the material using an electro-mechanical device to eject the sample from the analysis chamber.

19. The method of claim 18, further comprising providing an electric field to position the sample mixed with the material prior to removing the sample.

20. The method of claim 18, where the electro-mechanical device opens an exit port to eject the sample.