Methods and compositions for degrading pectin

• US 9,109,235 B2
• Filed: 07/15/2013
• Issued: 08/18/2015
• Est. Priority Date: 09/18/2008
• Status: Expired due to Fees

• Current Assignee: The University of Georgia Research Foundation, Inc.
• Original Assignee: The University of Georgia Research Foundation, Inc.
• Inventors: Peterson, Joy Bethune
• Primary Examiner(s): RAGHU, GANAPATHIRAM
• Application Number:
  US13/942,189
• Publication Number:
  US 20140004588A1
• Time in Patent Office: 764 Days
• Field of Search: 435/162, 435/161, 435/200, 435/201, 435/252.8, 435/252.3, 435/254.21, 435/254.11
• US Class Current: 1/1
• CPC Class Codes: C12N 9/2465 acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: C12P 7/14 Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
• CPC Class Codes: C12Y 302/01015 Polygalacturonase (3.2.1.15)
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present invention provides enriched polynucleotides, and enriched polypeptides having pectinase activity. The present invention also includes methods of using the polynucleotides and polypeptides described herein. For instance, the methods include producing metabolic product, such as ethanol.

Claims

1. A method for degrading pectin comprising:

contacting a composition comprising pectin with a composition comprising an enriched polypeptide, wherein the enriched polypeptide has pectinase activity under conditions suitable for the degradation of the pectin, wherein the polypeptide comprises an amino acid sequence, wherein the amino acid sequence and the amino acid sequence of SEQ ID NO;

4 have at least 80% identity, and wherein the pectin is degraded.

2. The method of claim 1 further comprising contacting the degraded pectin with a polypeptide having oligogalacturonate activity.

3. The method of claim 1 wherein the composition comprises a lignocellulosic material.

4. The method of claim 3 wherein the lignocellulose material is obtained from a fruit or a vegetable.

5. A method for degrading pectin comprising:

contacting a composition comprising pectin with a genetically modified microbe comprising an exogenous polynucleotide encoding a polypeptide having pectinase activity under conditions suitable for expression of the exogenous polypeptide and degradation of the pectin, wherein the polypeptide comprises an amino acid sequence, wherein the amino acid sequence and the amino acid sequence of SEQ ID NO;

4 have at least 80% identity, and wherein the pectin is degraded.

6. The method of claim 5 further comprising contacting the degraded pectin with a polypeptide having oligogalacturonate activity.

7. The method of claim 5 wherein the genetically modified microbe expresses an exogenous polypeptide having oligogalacturonate activity.

8. The method of claim 5 wherein the genetically modified microbe produces ethanol.

9. The method of claim 5 wherein the composition comprises a lignocellulosic material.

10. The method of claim 9 wherein the lignocellulose material is obtained from a fruit or a vegetable.

11. The method of claim 5 wherein the genetically modified microbe is a gram-negative microbe or a fungus.

12. The method of claim 5 wherein the genetically modified microbe is E. coli or S. cerevisiae.

13. The method of claim 5 wherein the polypeptide is enriched.