Microbe detection via hybridizing magnetic relaxation nanosensors
First Claim
Patent Images
1. A method of detecting a nucleic acid analyte of interest in a sample, the method comprising:
- (a) contacting nucleic acid from a sample with a hybridizing magnetic relaxation nanosensor (hMRS) comprising a plurality of nucleic acid sequence probes configured to specifically hybridize to one unique sequence of the nucleic acid analyte of interest, so that only one hMRS hybridizes per nucleic acid analyte of interest; and
(b) detecting a change in the magnetic resonance signal from a hMRS hybridized to a nucleic acid analyte of interest in the nucleic acid from the sample;
thereby detecting the presence of the nucleic acid analyte of interest in the sample.
2 Assignments
0 Petitions
Accused Products
Abstract
Disclosed herein are methods and materials for facilitating the detection of nucleic acid analytes of interest. Specifically exemplified herein are methods for detecting mycobacterial microorganisms, namely Mycobacterium avium spp. paratuberculosis. Also disclosed is new hybridizing magnetic relaxation nanosensor (hMRS) particularly adapted to detect a target nucleic acid analyte of interest.
17 Citations
18 Claims
-
1. A method of detecting a nucleic acid analyte of interest in a sample, the method comprising:
-
(a) contacting nucleic acid from a sample with a hybridizing magnetic relaxation nanosensor (hMRS) comprising a plurality of nucleic acid sequence probes configured to specifically hybridize to one unique sequence of the nucleic acid analyte of interest, so that only one hMRS hybridizes per nucleic acid analyte of interest; and (b) detecting a change in the magnetic resonance signal from a hMRS hybridized to a nucleic acid analyte of interest in the nucleic acid from the sample;
thereby detecting the presence of the nucleic acid analyte of interest in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
-
- 13. A hybridizing magnetic relaxation nanosensor (hMRS), comprising a magnetic nanoparticle associated with a plurality of nucleic acid probes configured to specifically hybridize to one unique sequence of a nucleic acid analyte of interest, so that only one hMRS hybridizes per nucleic acid analyte of interest.
Specification