Compositions and methods to detect;nucleic acid

• US 9,109,262 B2
• Filed: 03/01/2010
• Issued: 08/18/2015
• Est. Priority Date: 12/26/2007
• Status: Active Grant

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Inventors: Hogan, James J., Andruszkiewicz, Irene, Bungo, Jennifer J., Kaplan, Shannon K.
• Primary Examiner(s): HORLICK, KENNETH R
• Application Number:
  US12/715,132
• Publication Number:
  US 20100159530A1
• Time in Patent Office: 1,996 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae

Abstract

Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for Candida albicans 26S rRNA sequences or DNA encoding 26S rRNA. Methods are disclosed for detecting the presence of C. albicans in samples by using the disclosed compositions in methods that include in vitro nucleic acid amplification of a 26S rRNA sequence or DNA encoding the 26S rRNA sequence to produce a detectable amplification product.

Claims

1. A set of oligomers for use in amplifying a Candida albicans 26S rRNA sequence or DNA encoding the 26S rRNA sequence, the set of oligomers comprising first and second amplification oligomers, wherein the first amplification oligomer consists of the base sequence of SEQ ID NO:

1, the DNA equivalent of SEQ ID NO;

1, or a combination RNA/DNA equivalent of SEQ ID NO;

1, and wherein the second amplification oligomer is a promoter-primer comprising a 5′

promoter sequence and a target binding region consisting of the base sequence of SEQ ID NO;

6, the DNA equivalent of SEQ ID NO;

6, or a combination RNA/DNA equivalent of SEQ ID NO;

6.

2. The set of oligomers of claim 1, wherein the base sequence of the second amplification oligomer consists of the base sequence of SEQ ID NO:

7.

3. The set of oligomers of claim 1, further comprising a probe up to 50 bases in length and comprising a target binding sequence that includes at least 10 contiguous bases of the base sequence of SEQ ID NO:

10, the DNA equivalent of SEQ ID NO;

10, or a combination RNA/DNA equivalent of SEQ ID NO;

10.

4. The set of oligomers of claim 3, wherein the target binding sequence of the probe comprises the base sequence of SEQ ID NO:

10, the DNA equivalent of SEQ ID NO;

10, or a combination RNA/DNA equivalent of SEQ ID NO;

10.

5. The set of oligomers of claim 4, wherein the base sequence of the probe consists of the base sequence of SEQ ID NO:

11, the DNA equivalent of SEQ ID NO;

11, or a combination RNA/DNA equivalent of SEQ ID NO;

11.

6. The set of oligomers of claim 3, wherein the probe comprises a label.