Surface treatment

• US 9,110,056 B2
• Filed: 08/13/2009
• Issued: 08/18/2015
• Est. Priority Date: 12/20/2002
• Status: Expired due to Fees

• Current Assignee: International Business Machines Corporation
• Original Assignee: International Business Machines Corporation
• Inventors: Amontov, Sergey, Delamarche, Emmanuel, Michel, Bruno
• Primary Examiner(s): Wilder, Cynthia B
• Application Number:
  US12/540,526
• Publication Number:
  US 20090298161A1
• Time in Patent Office: 2,196 Days
• Field of Search: 435/287.2, 435/6.1
• US Class Current: 1/1
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

A biosensor comprising surface treated with a method for producing a monolayer of molecules on a surface, the method comprising loading a stamp with seed molecules, transferring seed molecules from the stamp to the surface, wherein transferring comprises transferring a fraction of the seed molecules loaded on the stamp to the surface and adsorbing the seed molecules to the stamp and adsorbing the seed molecules to the surface, the adsorption of the seed molecules to the stamp being stronger than the adsorption of the seed molecules to the surface, self-completing amplification of the seed molecules via an amplifying reaction to produce the monolayer on a flat surface, wherein self-completing amplification comprises producing a homogeneous area, wherein the homogeneous area comprises a monolayer of molecules on the surface, and wherein the monolayer of molecules on the surface has no diffusive component that can relocate and destroy amplification accuracy.

Claims

1. A biosensor comprising a flat surface encompassed by a homogeneous area comprising a monolayer of molecules with no diffusive seed molecules that can relocate and destroy amplification accuracy, the monolayer of molecules produced on the surface via a method comprising:

loading a stamp with seed molecules;

transferring seed molecules from the stamp to the flat surface, wherein the transferring comprises transferring a fraction of the seed molecules loaded on the stamp to the flat surface and wherein the transferring comprises adsorbing the seed molecules to the stamp and adsorbing the seed molecules to the flat surface, the adsorption of the seed molecules to the stamp being stronger than the adsorption of the seed molecules to the flat surface; and

self-completing amplification of the seed molecules via an amplifying reaction to produce the monolayer on the flat surface, wherein self-completing amplification of the seed molecules via an amplifying reaction to produce the monolayer comprises producing a homogeneous area extending over the entirety of the flat surface, wherein the homogeneous area comprises a monolayer of molecules formed from a single type of molecule.

2. A biosensor as claimed in claim 1, wherein in producing a monolayer of molecules on a surface, amplifying comprises linear amplification of the seed molecules.

3. A biosensor as claimed in claim 1, wherein in producing a monolayer of molecules on a surface, amplifying comprises exponential amplification of the seed molecules.

4. A biosensor as claimed in claim 1, wherein in producing a monolayer of molecules on a surface, amplifying comprises directional amplification of the seed molecules.

5. A biosensor as claimed in claim 4, wherein the seed molecules are directionally amplified to form conductive structures.

6. A biosensor as claimed in claim 4, comprising electroless plating of the directionally amplified seed molecules with a metal.

7. A biosensor as claimed in claim 4, wherein the directional amplification is controlled by the geometry of the seed molecule.

8. A biosensor as claimed in claim 4, wherein the directional amplification is controlled by application of an external force.

9. A biosensor as claimed in claim 8, wherein the external force comprises an electrical force.

10. A biosensor as claimed in claim 8, wherein the external force comprises a magnetic force.

11. A biosensor as claimed in claim 8, wherein the external force comprises a hydrodynamic force.

12. A biosensor as claimed in claim 1, wherein in producing a monolayer of molecules on a surface, amplifying comprises a polymerase chain reaction.

13. A biosensor as claimed in claim 12, wherein the polymerase chain reaction comprises binding at least one primer to the flat surface.

14. A biosensor as claimed in claim 13, wherein the polymerase chain reaction comprises supplying a primer in solution.

15. A biosensor as claimed in claim 1, wherein the monolayer protects the flat surface from etchants.

16. A biosensor as claimed in claim 1, wherein the monolayer comprises DNA.

17. A biosensor as claimed in claim 1, wherein producing a monolayer of molecules on a surface comprises repeating the transferring and amplifying on plural surfaces before reloading the stamp with seed molecules.