Moenomycin biosynthesis-related compositions and methods of use thereof
First Claim
Patent Images
1. An isolated recombinant cell expressing one or more polypeptides that comprise a moenomycin biosynthesis-related polypeptide selected from the group consisting of:
- MoeA4 (SEQ ID NO;
29), MoeB4 (SEQ ID NO;
30), MoeC4 (SEQ ID NO;
28), MoeB5 (SEQ ID NO;
27), Moe A5 (SEQ ID NO;
26), MoeD5 (SEQ ID NO;
47), MoeJ5 (SEQ ID NO;
48), MoeE5 (SEQ ID NO;
42), MoeF5 (SEQ ID NO;
36), MoeH5 (SEQ ID NO;
37), MoeK5 (SEQ ID NO;
38), MoeM5 (SEQ ID NO;
39), MoeN5 (SEQ ID NO;
43), MoeO5 (SEQ ID NO;
44), MoeX5 (SEQ ID NO;
46), MoeP5 (SEQ ID NO;
45), MoeR5 (SEQ ID NO;
40), MoeS5 (SEQ ID NO;
41), MoeGT1 (SEQ ID NO;
31), MoeGT2 (SEQ ID NO;
32), MoeGT3 (SEQ ID NO;
33), MoeGT4 (SEQ ID NO;
34), MoeGT5 (SEQ ID NO;
35), and variants thereof having at least about 95% sequence identity to the corresponding natural moenomycin biosynthesis-related polypeptide, wherein the cell is selected from the group consisting of;
Streptomyces lividans TK24, E. coli, a mammalian cell, a yeast cell, and an insect cell.
1 Assignment
0 Petitions
Accused Products
Abstract
The methods and compositions described herein relate to the identification, isolation, and characterization of genes which encode proteins useful for the biosynthesis of transglycosylase inhibitors such as moes. The methods and compositions also relate to the production of such proteins, and their use in the synthesis of moes, the expression of moes, and the production of modified moes.
-
Citations
12 Claims
-
1. An isolated recombinant cell expressing one or more polypeptides that comprise a moenomycin biosynthesis-related polypeptide selected from the group consisting of:
- MoeA4 (SEQ ID NO;
29), MoeB4 (SEQ ID NO;
30), MoeC4 (SEQ ID NO;
28), MoeB5 (SEQ ID NO;
27), Moe A5 (SEQ ID NO;
26), MoeD5 (SEQ ID NO;
47), MoeJ5 (SEQ ID NO;
48), MoeE5 (SEQ ID NO;
42), MoeF5 (SEQ ID NO;
36), MoeH5 (SEQ ID NO;
37), MoeK5 (SEQ ID NO;
38), MoeM5 (SEQ ID NO;
39), MoeN5 (SEQ ID NO;
43), MoeO5 (SEQ ID NO;
44), MoeX5 (SEQ ID NO;
46), MoeP5 (SEQ ID NO;
45), MoeR5 (SEQ ID NO;
40), MoeS5 (SEQ ID NO;
41), MoeGT1 (SEQ ID NO;
31), MoeGT2 (SEQ ID NO;
32), MoeGT3 (SEQ ID NO;
33), MoeGT4 (SEQ ID NO;
34), MoeGT5 (SEQ ID NO;
35), and variants thereof having at least about 95% sequence identity to the corresponding natural moenomycin biosynthesis-related polypeptide, wherein the cell is selected from the group consisting of;
Streptomyces lividans TK24, E. coli, a mammalian cell, a yeast cell, and an insect cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
- MoeA4 (SEQ ID NO;
Specification