Transposon end compositions and methods for modifying nucleic acids
First Claim
1. A population of non-selectively amplified linear DNA fragments representative of a target genomic DNA, wherein the ends of each DNA fragment comprise a transposon polynucleotide, wherein the transposon polynucleotide comprises a sequencing tag and a transposon end sequence, wherein the DNA fragments are attached to a surface through capture tags and are obtained using a plurality of transposome complexes for next generation sequencing, wherein(1) at least one transposome complex comprises a hyperactive Tn5 transposase and at least a first transposon polynucleotide comprising a Tn5-type transposon end sequence and a sequencing tag;
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Accused Products
Abstract
Compositions of transposome complexes for generating DNA fragments with specific 5′- and 3′-tags. Kits for generating libraries for sequencing, with transposome complexes, enzymes, oligonucleotides or other components.
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Citations
27 Claims
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1. A population of non-selectively amplified linear DNA fragments representative of a target genomic DNA, wherein the ends of each DNA fragment comprise a transposon polynucleotide, wherein the transposon polynucleotide comprises a sequencing tag and a transposon end sequence, wherein the DNA fragments are attached to a surface through capture tags and are obtained using a plurality of transposome complexes for next generation sequencing, wherein
(1) at least one transposome complex comprises a hyperactive Tn5 transposase and at least a first transposon polynucleotide comprising a Tn5-type transposon end sequence and a sequencing tag; - and
(2) at least one transposome complex comprises a hyperactive Tn5 transposase and at least a second transposon polynucleotide comprising a Tn5-type transposon end sequence and a sequencing tag. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 24)
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- 14. A library for next generation sequencing comprising a population of non-selectively amplified linear nucleic acid fragments representative of a target nucleic acid and attached through a capture tag to a surface, wherein the ends of each nucleic acid fragment comprise a sequencing tag and a transposon polynucleotide end sequence.
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26. A population of non-selectively amplified linear DNA fragments for next generation sequencing that is representative of the sequences of both strands of a target genomic DNA from which the DNA fragments were generated, wherein both ends of each DNA fragment comprise a transferred transposon end sequence and wherein at least one end of each DNA fragment also comprises a sequencing tag, wherein the DNA fragments are attached to a surface through capture tags and are produced using a plurality of transposome complexes, wherein
(1) at least one transposome complex comprises a transposase and a transposon end sequence comprising a first transferred transposon end oligonucleotide; - and
(2) at least one transposome complex comprises a transposase and a transposon end sequence comprising a second transferred transposon end oligonucleotide. - View Dependent Claims (27)
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Specification