Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use
First Claim
Patent Images
1. A method of detecting the presence or absence of double stranded DNA in a sample, the method comprising:
- providing a sample suspected of containing double stranded DNA;
contacting the sample with a chemical compound to prepare a test sample;
illuminating the test sample with energy; and
detecting emission of energy from the test sample;
wherein the chemical compound has the structure;
0 Assignments
0 Petitions
Accused Products
Abstract
Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.
-
Citations
6 Claims
-
1. A method of detecting the presence or absence of double stranded DNA in a sample, the method comprising:
-
providing a sample suspected of containing double stranded DNA; contacting the sample with a chemical compound to prepare a test sample; illuminating the test sample with energy; and detecting emission of energy from the test sample; wherein the chemical compound has the structure; - View Dependent Claims (2, 3, 4, 5, 6)
-
Specification