Sensing method of electrochemical sensor

• US 9,116,113 B2
• Filed: 12/26/2012
• Issued: 08/25/2015
• Est. Priority Date: 08/10/2012
• Status: Active Grant

• Current Assignee: I-Shou University
• Original Assignee: I-Shou University
• Inventors: Wu, Jau-Yann, Wang, Shih-Han
• Primary Examiner(s): Ball, J. Christopher
• Application Number:
  US13/726,632
• Publication Number:
  US 20140042036A1
• Time in Patent Office: 972 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: G01N 27/305 optically transparent or photoresponsive electrodes
• CPC Class Codes: G01N 27/3277 being a redox reaction, e.g. detection by cyclic voltammetry voltammetry per se G01N27/42, G01N27/48
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72

Abstract

A sensing method of an electrochemical sensor including the following steps is provided. First, a fluorescent material is immobilized on a surface of an electrode so as to form a sensing electrode. The sensing electrode is then used to execute an electrochemical test of a target in a light-shielding environment.

Claims

1. A sensing method of an electrochemical sensor, the sensing method comprising:

immobilizing a fluorescent material on a surface of an electrode to form a sensing electrode, wherein the fluorescent material is a material having at least one nitrogen atom;

in a light-shielding environment, using the sensing electrode to perform an electrochemical test on a target.

2. The sensing method of claim 1, wherein the target includes an electroactive substance.

3. The sensing method of claim 2, wherein the electroactive substance includes metal ions or quinone.

4. The sensing method of claim 2, wherein the electroactive substance includes reduced β

-nicotinamide adenine dinucleotide (NADH) or hydrogen peroxide (H₂O₂).

5. The sensing method of claim 1, wherein the electrochemical test includes a voltammetric sensing mode.

6. The sensing method of claim 1, wherein the technique for immobilizing the fluorescent material on the surface of the electrode includes covalent bond formation dependent or non-covalent bond formation dependent.

7. The sensing method of claim 1, wherein the fluorescent material comprises quinine hemisulfate.

8. The sensing method of claim 1, wherein the fluorescent material comprises an oligochitosan fluorescent derivative.

9. The sensing method of claim 1, wherein the fluorescent material comprises a polyethylene imine (PEI) fluorescent derivative.