Quantitative determination method for target particles, photometric analysis device, and computer program for photometric analysis

• US 9,116,127 B2
• Filed: 10/03/2013
• Issued: 08/25/2015
• Est. Priority Date: 04/18/2011
• Status: Active Grant

• Current Assignee: Olympus Corporation
• Original Assignee: Olympus Corporation
• Inventors: Nakata, Hidetaka
• Primary Examiner(s): PHAM, HOA Q
• Application Number:
  US14/045,002
• Publication Number:
  US 20140099630A1
• Time in Patent Office: 691 Days
• Field of Search: 356335-343, 356432-440, 3562431-2438, 250/459.1, 250/458.1, 435/6.11, 435/5, 435/7.1
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 2537/157 A reaction step characterised by the number of molecules incorporated or released
• CPC Class Codes: C12Q 2545/114 involving a quantitation step
• CPC Class Codes: C12Q 2565/601 being a microscope, e.g. atomic force microscopy [AFM]
• CPC Class Codes: G01N 2021/6419 Excitation at two or more wavelengths
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 2021/6432 Quenching
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence

Abstract

The method of the present invention includes: preparing a sample solution containing the target particles and luminescent probes to be bound to the target particles, and binding these in the sample solution; moving a position of a light detection region of the optical system in the sample solution using a confocal microscope or a multiphoton microscope, and detecting light signal emitted from the luminescent probe in the light detection region while moving the position of the light detection region, and individually detecting the target particles directly or indirectly; and counting the number of the detected target particles, and calculating the concentration of the target particles in the sample solution from the number of the counted target particles on the basis of a calibration curve that approximates the correlation between the concentration or quantity of the target particles in the sample solution and the number of the target particles.

Claims

1. A method for quantifying target particles which are dispersed and moving at random in a sample solution, the method comprising:

(a) preparing a sample solution containing the target particles and luminescent probes to be bound to the target particles, and binding the target particles and the luminescent probes in the sample solution;

(b) moving a position of a light detection region of an optical system in the sample solution with use of an optical system of a confocal microscope or a multiphoton microscope, and detecting a light signal emitted from the luminescent probes in the light detection region while moving the position of the light detection region of the optical system in the sample solution, and individually detecting the target particle directly or indirectly; and

(c) counting, using a processor, the number of the target particles detected in the (b) and calculating, using the processor, the concentration of the target particles in the sample solution from the number of the counted target particles on the basis of a calibration curve that approximates the correlation between the concentration or quantity of the target particles in the sample solution and the number of the target particles.

2. The method for quantifying target particles according to claim 1, wherein

the position of the light detection region is moved at a predetermined speed, in the moving step.

3. The method for quantifying target particles according to claim 1, wherein

the position of the light detection region is moved at a speed higher than the speed of diffusional movement of either quicker ones of the luminescent probes or the target particles bound to the luminescent probes, in the moving step.

4. The method for quantifying target particles according to claim 1, wherein

it is detected that the luminescent probe or the target particle bound to the luminescent probe has entered the light detection region, based on the shape of the chronological detected light signal, in the detection step.

5. The method for quantifying target particles according to claim 1, wherein:

the luminescent probe has an energy donor site and an energy acceptor site, which produce a fluorescence energy transfer phenomenon when these sites are close to each other;

the distance between the energy donor site and the energy acceptor site is different between a binding state where the luminescent probe is bound to the target particle and a non-binding state where the luminescent probe is not bound to the target particle; and

an emission characteristic of light emitted from the luminescent probe is different between the binding state and the non-binding state.

6. The method for quantifying target particles according to claim 1, wherein:

the target particle is a nucleic acid; and

the luminescent probe is a single stranded nucleic acid which is specifically hybridizable with the target particle, and which is bound with at least either one of a fluorescent substance serving as an energy donor and a substance serving as an energy acceptor in a fluorescence energy transfer phenomenon.

7. The method for quantifying target particles according to claim 1, further comprising:

(d) separating a luminescent probe which is not bound to the target particle from the sample solution, and collecting a complex including the target particle and the luminescent probe, after the (a).

8. The method for quantifying target particles according to claim 7, further comprising:

(e) dissociating the luminescent probe from the complex having been collected in the (d), thereafter separating the free luminescent probe and the target particle from each other, and collecting them.

9. A photometric analysis device for detecting light from luminescent particles dispersed and moving at random in a sample solution, with use of an optical system of a confocal microscope or a multiphoton microscope, the device comprising:

a light detection region mover for moving a position of a light detection region of the optical system in the sample solution by changing an optical path of the optical system;

a light detector for detecting light from the luminescent particle in the light detection region;

a signal processor for individually detecting a light signal of the light emitted from respective ones of the luminescent particles detected in the light detector while moving the position of the light detection region in the sample solution, and counting the number of the luminescent particles detected during the moving of the position of the light detection region by counting the number of the individually detected light signals from the luminescent particle;

a storage for storing a calibration curve that approximates the correlation between the concentration or quantity of the luminescent particles in the sample solution and the number of the luminescent particles counted from the sample solution;

a concentration calculator for, on the basis of the calibration curve, calculating the concentration of the luminescent particles in the sample solution from the number of the luminescent particles counted in the signal processor; and

a display for displaying the concentration of the luminescent particles in the sample solution calculated by the concentration calculator.

10. The device according to claim 9, wherein

the light detection region mover moves the position of the light detection region at a predetermined speed.

11. The device according to claim 9, wherein

the light detection region mover moves the position of the light detection region at a speed higher than the speed of diffusional movement of the luminescent particle.

12. The device according to claim 9, wherein

the signal processor detects that one of the luminescent particles has entered the light detection region, based on the shape of the chronological detected light signal in the light detector.

13. The device according to claim 12, wherein

the signal processor detects that one of the luminescent particles has entered the light detection region, when the light signal having an intensity greater than a predetermined threshold value is detected.

14. The device according to claim 9, wherein

the signal processor determines the number density or concentration of the luminescent particles in the sample solution, on the basis of the number of the detected luminescent particles.

15. A non-transitory computer readable storage device having a computer program product including programmed instructions for photometric analysis of detecting light from a luminescent particle dispersed and moving at random in a sample solution, with use of an optical system of a confocal microscope or a multiphoton microscope, the programmed instructions causing a computer to perform the steps comprising:

changing the optical path of the optical system, so as to move a position of a light detection region of the optical system in the sample solution;

detecting the light from the luminescent particle in the light detection region, while moving the position of the light detection region in the sample solution;

individually detecting a light signal of the light emitted from respective ones of the detected luminescent particle;

counting the number of the luminescent particles detected during the moving of the position of the light detection region by counting the number of the individually detected light signals of the light from the luminescent particles; and

calculating the concentration of the target particles in the sample solution from the counted number of the luminescent particles on the basis of a calibration curve that approximates the correlation between the concentration or quantity of the luminescent particles in the sample solution and the number of the luminescent particles counted from the sample solution.

16. The non-transitory computer readable storage device according to claim 15, wherein

the position of the light detection region is moved at a predetermined speed, in the changing step.

17. The non-transitory computer readable storage device according to claim 15, wherein

the position of the light detection region is moved at a speed higher than the speed of diffusional movement of the luminescent particle, in the changing step.

18. The non-transitory computer readable storage device according to claim 15, wherein

it is detected that one of the luminescent particles has entered the light detection region, based on the shape of the chronological detected light signal, in the light signal detection step.

19. The non-transitory computer readable storage device according to claim 15, wherein

it is detected that one of the luminescent particles has entered the light detection region, when a light signal having an intensity greater than a predetermined threshold value is detected, in the light signal detection step.

20. The non-transitory computer readable storage device according to claim 15, further comprising a procedure for determining the number density or concentration of the luminescent particles in the sample solution, on the basis of the number of the detected luminescent particles.