Fluid delivery system and method
First Claim
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1. A method comprising:
- providing a first fluid and a second fluid maintained separately from each other by a third fluid in a common, sealed vessel, wherein the third fluid is adjacent to and substantially immiscible with the first and second fluids;
unsealing the vessel;
transferring the first, third and second fluids in series from the vessel to an analysis region; and
avoiding contact between at least portions of the first and second fluids, at least until after the fluids have been applied to the analysis region.
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Abstract
A method and apparatus for delivering one or more fluids. Fluids may be delivered sequentially from a common vessel to a chemical, biological or biochemical process.
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Citations
35 Claims
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1. A method comprising:
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providing a first fluid and a second fluid maintained separately from each other by a third fluid in a common, sealed vessel, wherein the third fluid is adjacent to and substantially immiscible with the first and second fluids; unsealing the vessel; transferring the first, third and second fluids in series from the vessel to an analysis region; and avoiding contact between at least portions of the first and second fluids, at least until after the fluids have been applied to the analysis region. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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2. A method comprising:
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providing an analysis region; providing a first fluid and a second fluid maintained separately from each other by a third fluid adjacent to and substantially immiscible with the first and second fluids in a common, sealed vessel, wherein the vessel and the analysis region are formed in a microfluidic chip; unsealing the vessel; applying in series the first, third, and second fluids to the analysis region; and avoiding contact between at least portions of the first and second fluids, at least until after the fluids have been applied to the analysis region.
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3. A fluidic system comprising:
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an analysis region; and a sealed vessel containing a first fluid, a second fluid, and a third fluid, wherein the third fluid is adjacent to and substantially immiscible with the first and second fluids, wherein the sealed vessel is adapted and arranged to be unsealed such that the fluids from the vessel can be applied to the analysis region, and wherein the analysis region and the vessel are formed in a microfluidic chip. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35)
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Specification