Method for controlling herbicide-resistant plants
First Claim
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1. A method for selectively controlling targeted herbicide-resistant weeds or volunteer plants comprising topically applying onto a surface of said weeds or volunteer plants one or more polynucleotides and a transfer agent in a manner that does not integrate said one or more polynucleotides into a chromosome of said weeds or volunteer plants;
- wherein said transfer agent is selected from the group consisting of an organosilicone surfactant and a cationic lipid;
wherein said transfer agent enables said one or more polynucleotides to permeate from the surface of said weeds or volunteer plants into cells of said weeds or volunteer plants;
wherein said one or more polynucleotides comprise a sequence essentially identical or essentially complementary to a coding or non-coding sequence of an endogenous gene of said weed or volunteer plant or a messenger RNA that is transcribed from an endogenous gene of said weed or volunteer plant; and
wherein said endogenous gene;
(i) is an essential gene for maintaining the growth or life of said weed or volunteer plant, (ii) encodes a protein that provides herbicide resistance to said weed or volunteer plant, or (iii) transcribes to an RNA regulatory agent.
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Abstract
This invention provides polynucleotide molecules and methods for regulating genes in plants, e.g., by providing RNA for systemic regulation of genes. Various aspects of the invention provide polynucleotide molecules and methods for regulating endogenous genes and transgenes in a plant cell and polynucleotide molecules.
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Citations
30 Claims
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1. A method for selectively controlling targeted herbicide-resistant weeds or volunteer plants comprising topically applying onto a surface of said weeds or volunteer plants one or more polynucleotides and a transfer agent in a manner that does not integrate said one or more polynucleotides into a chromosome of said weeds or volunteer plants;
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wherein said transfer agent is selected from the group consisting of an organosilicone surfactant and a cationic lipid; wherein said transfer agent enables said one or more polynucleotides to permeate from the surface of said weeds or volunteer plants into cells of said weeds or volunteer plants; wherein said one or more polynucleotides comprise a sequence essentially identical or essentially complementary to a coding or non-coding sequence of an endogenous gene of said weed or volunteer plant or a messenger RNA that is transcribed from an endogenous gene of said weed or volunteer plant; and wherein said endogenous gene;
(i) is an essential gene for maintaining the growth or life of said weed or volunteer plant, (ii) encodes a protein that provides herbicide resistance to said weed or volunteer plant, or (iii) transcribes to an RNA regulatory agent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification