Non-natural amino acid replication-dependent microorganisms and vaccines
First Claim
1. A non-human animal vaccine, comprising a Mycobacterium avium subspecies paratuberculosis (MAP) cell and an adjuvant, wherein the MAP cell comprises a nucleotide sequence encoding an essential gene product that is required for replication, wherein the nucleotide sequence comprises a selector codon that codes for site-specific incorporation of a non-natural amino acid into the essential gene product, such as that the MAP cell replicates only in the presence of the said non-natural amino acid, and has limited or no replication capability in the absence of said non-natural amino acid.
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Accused Products
Abstract
Compositions and methods of producing vaccines, including methods wherein whole organism vaccines are provided with limited replication abilities, thereby increasing vaccine safety and efficacy, through the use of non-natural, unnatural, or non-naturally encoded amino acids.
168 Citations
8 Claims
- 1. A non-human animal vaccine, comprising a Mycobacterium avium subspecies paratuberculosis (MAP) cell and an adjuvant, wherein the MAP cell comprises a nucleotide sequence encoding an essential gene product that is required for replication, wherein the nucleotide sequence comprises a selector codon that codes for site-specific incorporation of a non-natural amino acid into the essential gene product, such as that the MAP cell replicates only in the presence of the said non-natural amino acid, and has limited or no replication capability in the absence of said non-natural amino acid.
Specification