Linear valve arrays
First Claim
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1. A method, comprising:
- (a) providing a microfluidic apparatus comprising;
(i) a valve array comprising a series of N valves connected through unbranched fluidic connections, the series comprising, in order, a first terminal valve, at least three intermediate valves and a second terminal valve;
(ii) at least one first port, each first port fluidically connected through a separate control valve to the first terminal valve; and
(iii) at least one second port, each second port fluidically connected through a separate control valve to the second terminal valve;
and wherein the terminal valves and intermediate valves are diaphragm valves each comprising a valve chamber; and
(b) performing a biochemical reaction by;
introducing a target sample from one of the ports into a valve chamber of a first diaphragm valve in the series;
introducing a first reagent from one of the ports into a valve chamber of a second diaphragm valve in the series adjacent to the first diaphragm valve in the series; and
mixing said target sample and said first reagent within said microfluidic apparatus to form a mixed reaction, wherein said mixing comprises moving both said first reagent and said target sample from said valve chambers of the first and second diaphragm valves in the series to a chamber of at least a third diaphragm valve of the series and flowing at least one of said first reagent and said target sample in the valve series in a forward and in a reverse direction; and
allowing a biochemical reaction to occur to form a product solution.
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Abstract
The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.
502 Citations
17 Claims
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1. A method, comprising:
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(a) providing a microfluidic apparatus comprising; (i) a valve array comprising a series of N valves connected through unbranched fluidic connections, the series comprising, in order, a first terminal valve, at least three intermediate valves and a second terminal valve; (ii) at least one first port, each first port fluidically connected through a separate control valve to the first terminal valve; and (iii) at least one second port, each second port fluidically connected through a separate control valve to the second terminal valve; and wherein the terminal valves and intermediate valves are diaphragm valves each comprising a valve chamber; and (b) performing a biochemical reaction by; introducing a target sample from one of the ports into a valve chamber of a first diaphragm valve in the series; introducing a first reagent from one of the ports into a valve chamber of a second diaphragm valve in the series adjacent to the first diaphragm valve in the series; and mixing said target sample and said first reagent within said microfluidic apparatus to form a mixed reaction, wherein said mixing comprises moving both said first reagent and said target sample from said valve chambers of the first and second diaphragm valves in the series to a chamber of at least a third diaphragm valve of the series and flowing at least one of said first reagent and said target sample in the valve series in a forward and in a reverse direction; and allowing a biochemical reaction to occur to form a product solution. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification