×

Rapid screening of biologically active nucleases and isolation of nuclease-modified cells

  • US 9,121,072 B2
  • Filed: 09/15/2011
  • Issued: 09/01/2015
  • Est. Priority Date: 09/27/2007
  • Status: Active Grant
First Claim
Patent Images

1. A host cell comprising an endogenous genome and an episomal reporter construct, the episomal reporter construct comprising a sequence consisting essentially of target sequences for multiple pairs of zinc finger nucleases, the target sequences flanked by sequences encoding a disabled reporter gene, wherein the target sequences are the same as sequences in the endogenous genome and further wherein the sequences encoding the disabled reporter comprise identical repeated sequences flanking the target sequences.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×