Methods and compositions for improving the appearance of skin

• US 9,125,843 B2
• Filed: 10/03/2013
• Issued: 09/08/2015
• Est. Priority Date: 10/03/2013
• Status: Active Grant

• Current Assignee: ELC Management LLC (The Estee Lauder Companies Inc.)
• Original Assignee: ELC Management LLC (The Estee Lauder Companies Inc.)
• Inventors: Pernodet, Nadine A., Collins, Donald F.
• Primary Examiner(s): Ha, Julie
• Assistant Examiner(s): KOMATSU, LI N
• Application Number:
  US14/045,075
• Publication Number:
  US 20150098963A1
• Time in Patent Office: 705 Days
• US Class Current: 1/1
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/9711 Phaeophycota or Phaeophyta [brown algae], e.g. Fucus
• CPC Class Codes: A61K 8/9728 Fungi, e.g. yeasts
• CPC Class Codes: A61K 8/9794 Liliopsida [monocotyledons]
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/16 Emollients or protectives, e.g. against radiation
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61Q 19/08 Anti-ageing preparations

Abstract

A method for stimulating collagen synthesis in aging skin cells in need of treatment by topically applying a composition comprising at least one extract from Laminaria genus, at least one extract from the Narcissus genus, and at least one peptide that stimulates sirtuin 6 activity in the form of a peptide or a yeast extract where the peptide is component of the extract; and a treatment composition comprising at least one extract from Laminaria genus, at least one extract from the Narcissus genus; and at least one peptide that stimulates sirtuin 6 activity.

Claims

1. A topical emulsion composition comprising:

from 0.0001 to 5% at least one Laminaria digitata extract consisting of an aqueous extraction and leaching of lyophilized algae, having a laminarin or mannitol or both laminarin and mannitol content of about 1 to 2% by weight of the total Laminaria digitata composition;

from 0.001 to 5% of at least one Narcissus tazetta extract consisting of a water soluble extract filtrate of Narcissus tazetta bulbs in the dormant state; and

at least one synthetic peptide present in an amount from 0.0001 to 3% and selected from the group consisting of;

Glu-Ile-His-Gly-Ser-Leu-Phe-Lys-NH₂ (SEQ ID No;

     1),

His-Gly-Ser-Leu-Phe-Lys-NH₂ (SEQ ID No;

     2),

Leu-Val-Gly-Ala-Gly-Val-Ser-Ala-NH₂ (SEQ ID No;

     3),

Gly-Ala-Gly-Val-Ser-Ala-Glu (SEQ ID No;

     4),

Gly-Ala-Gly-Val-Ser-Ala-Glu-NH₂ (SEQ ID No;

     5),

Thr-Gln-Asn-Ile-Asp-Glu-Leu (SEQ ID No;

     6),

Thr-Gln-Asn-Ile-Asp-Glu-Leu-NH₂ (SEQ ID No;

     7),

Val-Ile-Thr-Gln-Asn-Ile-Asp-Ala-NH₂ (SEQ ID No;

     8);

and mixtures thereof, with all percentages by weight of the total composition.

2. The composition of claim 1, wherein the peptide is in the form of a mixture with yeast extract.

3. The composition of claim 1, wherein the Laminaria digitata extract is present in an amount ranging from 0.0001 to 1%.

4. The composition of claim 1, wherein the Narcissus tazetta extract is present in an amount ranging from 0.001 to 1.5%.

5. The composition of claim 1 in the form of a serum, cream, or lotion.

6. The composition of claim 1, wherein the peptide is selected from the group consisting of:

Gly-Ala-Gly-Val-Ser-Ala-Glu-NH₂ (SEQ ID No;

     5),

Thr-Gln-Asn-Ile-Asp-Glu-Leu-NH₂ (SEQ ID No;

     7); and

mixtures thereof.

7. The composition of claim 1, wherein the Laminaria digitata extract is obtained by aqueous extraction and leaching of lyophilized algae to obtain a microfiltrate.

8. The composition of claim 1, wherein the peptide is present in

a yeast extract composition in an amount ranging from 10 to 1,000,000 ppm.

9. The composition of claim 1, wherein the Narcissus tazetta extract is in the form of a composition containing 62.5 parts glycerin, 37 parts water, and 0.5 parts Narcisuss tazetta bulb extract;

wherein the Laminaria digitata extract is in the form of a composition containing 91 parts water, 8 parts Laminaria digitata extract, and 1 part preservative;

the peptide is supplied to the composition in the form of a mixture with yeast extract, wherein the concentration of peptide in the yeast extract composition ranges from 10 to 1,000,000 ppm; and

wherein the composition synergistically increases collagen synthesis.

10. The composition of claim 9 in the form of a serum, cream, or lotion.

12. A method for stimulating collagen synthesis in aging skin cells in need of such treatment, the method comprising topically applying the composition according to claim 1.

13. The method of claim 1, wherein the stimulation of collagen synthesis causes a reduction in skin laxity, a reduction in the appearance of lines or wrinkles, or both.

14. The method of claim 1, wherein the topical composition is applied once or twice per day.

15. The method of claim 1, wherein the topical composition is applied before a period of sustained rest.

16. The method of claim 1, wherein the peptide is

Gly-Ala-Gly-Val-Ser-Ala-Glu-NH₂ (SEQ ID No:

5).

17. The method of claim 1, wherein the peptide is in a mixture with yeast extract.

11. A topical emulsion composition comprising:

from 0.0001 to 1% at least one extract of Laminaria digitata consisting of an extract from the aqueous extraction and leaching of lyophilized algae having a laminarin or mannitol or laminarin and mannitol content from about 1 to 2% by weight of the total Laminaria digitata extract;

from 0.001 to 1.5% of at least one extract of Narcissus tazetta consisting of a water soluble extract filtrate of Narcissus tazetta bulbs in the dormant state; and

at least one synthetic peptide present in an amount from 0.0001 to 1% and selected from the group consisting of;

Glu-Ile-His-Gly-Ser-Leu-Phe-Lys-NH₂ (SEQ ID No;

     1),

His-Gly-Ser-Leu-Phe-Lys-NH₂ (SEQ ID No;

     2),

Leu-Val-Gly-Ala-Gly-Val-Ser-Ala-NH₂ (SEQ ID No;

     3),

Gly-Ala-Gly-Val-Ser-Ala-Glu (SEQ ID No;

     4),

Gly-Ala-Gly-Val-Ser-Ala-Glu-NH₂ (SEQ ID No;

     5),

Thr-Gln-Asn-Ile-Asp-Glu-Leu (SEQ ID No;

     6),

Thr-Gln-Asn-Ile-Asp-Glu-Leu-NH₂ (SEQ ID No;

     7),

Val-Ile-Thr-Gln-Asn-Ile-Asp-Ala-NH₂ (SEQ ID No;

     8);

and mixtures thereof, with all percentages by weight of the total composition wherein said peptide is in a mixture with yeast extract.