Centrifuge configurations

• US 9,128,015 B2
• Filed: 09/09/2014
• Issued: 09/08/2015
• Est. Priority Date: 09/25/2011
• Status: Active Grant

• Current Assignee: Labrador Diagnostics LLC (FIG LLC (d/b/a Fortress Investment Group LLC))
• Original Assignee: Theranos Incorporated
• Inventors: Holmes, Elizabeth A., Frankovich, John Kent
• Primary Examiner(s): KWAK, DEAN P
• Application Number:
  US14/480,960
• Publication Number:
  US 20150125945A1
• Time in Patent Office: 364 Days
• Field of Search: 422/72, 422/415, 494/1, 494/16, 494/20, 494/21, 494/33, 494/34, 494/43, 494/60, 494/63, 494/80
• US Class Current: 1/1
• CPC Class Codes: B04B 13/00 Control arrangements specially designed for centrifuges; Programme control of centrifuges control arrangements for feed, charge, or discharge B04B11/00
• CPC Class Codes: B04B 5/0414 comprising test tubes
• CPC Class Codes: B04B 5/0421 pivotably mounted
• CPC Class Codes: B04B 7/08 Rotary bowls centrifugal casting machines B22D
• CPC Class Codes: B04B 9/04 Direct drive
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: G01N 1/40 Concentrating samples
• CPC Class Codes: G01N 1/4077 by other techniques involving separation of suspended solids
• CPC Class Codes: G01N 2001/4083 sedimentation

Abstract

Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.

Claims

1. A sample processing device comprising:

a programmable fluid handling system;

a cartridge receiving location accessible by the programmable fluid handling system;

at least one optical detector positioned at a location accessible by the programmable fluid handling system;

a centrifuge positioned at a location accessible by the programmable fluid handling system, wherein the centrifuge comprises;

a motor assembly comprising a rotor configured to rotate about a stator about an axis of rotation, wherein the motor assembly has a height that is oriented along the direction of the axis of rotation, wherein along said height said stator is circumscribed by said rotor; and

a base affixed to the rotor, wherein the base rotates about the stator, wherein the base comprises at least one bucket that is characterized in that (a) the bucket comprises a cavity to receive a sample vessel and (b) the bucket is configured to pivot about a pivot axis to move the cavity from a first orientation when the base is at rest to a second orientation when the base is rotating, wherein the bucket at a fully extended orientation does not extend beyond a circumferential perimeter of the base, and wherein the centrifuge is capable of achieving a rotational speed of at least about 1,000 RPM and wherein an entire bottom surface of the centrifuge is about 200 cm² or less.

2. The centrifuge of claim 1, wherein the cavity is configured to accept and confine a sample having a volume less than about 10 μ

L, thereby permitting centrifugation of said sample.

3. The centrifuge of claim 1, wherein the bucket includes a mass insert.

4. The centrifuge of claim 3, wherein the mass insert is made of a material of a mass greater than one or more other portion of the bucket.

5. The centrifuge of claim 3, wherein a force is exerted on the mass insert to retain the mass insert at a first position when the base is at rest.

6. The centrifuge of claim 5, wherein the force is exerted by a spring.

7. The centrifuge of claim 1, wherein the bucket has a center of mass below the pivot axis.

8. The centrifuge of claim 7, wherein the center of mass is further from the axis orthogonal to the bottom surface than the pivot.

9. The centrifuge of claim 1, wherein the cavity is provided in a module that is removable from the base.

10. The centrifuge of claim 1, wherein the height of the motor assembly comprising the rotor and stator is no greater than twice a height of the base.

11. The centrifuge of claim 1, wherein the sample vessel is configured to accept and confine a sample of no more than about 200 μ

L.

12. The centrifuge of claim 1, wherein the stator and a rotor portion that circumscribes the stator are configured to interact to cause rotor rotation.

13. The centrifuge of claim 1, wherein said centrifuge has a height of 5 cm or less.

14. The centrifuge of claim 13, wherein a greatest dimension of the centrifuge is 15 cm or less.