Systems and methods for detection of cells using engineered transduction particles
First Claim
1. A method, comprising:
- coupling a reagent module to a sample container such that a reaction chamber defined by the sample container is fluidically isolated from an exterior volume, the reaction chamber containing a sample, a seal surface of the reagent module being in a fixed location relative to the sample container after the coupling;
disposing, after the coupling, at least a distal end portion of the sample container into an instrument; and
actuating the instrument to;
produce a force on the reagent module to (1) move at least the distal end portion of the sample container into a detection volume of the instrument, and (2) place the seal surface into contact with a corresponding surface of the instrument to optically isolate the detection volume;
cause an optical assembly to receive, when the distal end portion of the sample container is in the detection volume, a signal associated with a magnitude of light emission in the detection volume; and
manipulate, during the receiving of the signal, the reagent module to convey a reagent into the reaction chamber of the sample container.
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Abstract
Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.
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Citations
17 Claims
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1. A method, comprising:
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coupling a reagent module to a sample container such that a reaction chamber defined by the sample container is fluidically isolated from an exterior volume, the reaction chamber containing a sample, a seal surface of the reagent module being in a fixed location relative to the sample container after the coupling; disposing, after the coupling, at least a distal end portion of the sample container into an instrument; and actuating the instrument to; produce a force on the reagent module to (1) move at least the distal end portion of the sample container into a detection volume of the instrument, and (2) place the seal surface into contact with a corresponding surface of the instrument to optically isolate the detection volume; cause an optical assembly to receive, when the distal end portion of the sample container is in the detection volume, a signal associated with a magnitude of light emission in the detection volume; and manipulate, during the receiving of the signal, the reagent module to convey a reagent into the reaction chamber of the sample container. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification