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Systems and methods for detection of cells using engineered transduction particles

  • US 9,133,497 B2
  • Filed: 02/02/2015
  • Issued: 09/15/2015
  • Est. Priority Date: 03/13/2013
  • Status: Active Grant
First Claim
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1. A method, comprising:

  • coupling a reagent module to a sample container such that a reaction chamber defined by the sample container is fluidically isolated from an exterior volume, the reaction chamber containing a sample, a seal surface of the reagent module being in a fixed location relative to the sample container after the coupling;

    disposing, after the coupling, at least a distal end portion of the sample container into an instrument; and

    actuating the instrument to;

    produce a force on the reagent module to (1) move at least the distal end portion of the sample container into a detection volume of the instrument, and (2) place the seal surface into contact with a corresponding surface of the instrument to optically isolate the detection volume;

    cause an optical assembly to receive, when the distal end portion of the sample container is in the detection volume, a signal associated with a magnitude of light emission in the detection volume; and

    manipulate, during the receiving of the signal, the reagent module to convey a reagent into the reaction chamber of the sample container.

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