Renovation and repopulation of decellularized tissues and cadaveric organs by stem cells

• US 9,139,813 B2
• Filed: 04/15/2013
• Issued: 09/22/2015
• Est. Priority Date: 02/14/2001
• Status: Expired due to Term

• Current Assignee: Celularity, Inc.
• Original Assignee: Anthrogenesis Corporation (Bristol-Myers Squibb Company)
• Inventors: Hariri, Robert J.
• Primary Examiner(s): LI, QIAN JANICE
• Application Number:
  US13/863,308
• Publication Number:
  US 20130230497A1
• Time in Patent Office: 890 Days
• US Class Current: 1/1
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 35/50 Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
• CPC Class Codes: A61L 2430/34 for soft tissue reconstruction
• CPC Class Codes: A61L 27/3695 characterised by the function or physical properties of the final product, where no specific conditions are defined to achieve this A61L27/3687, A61L27/3691 take precedence
• CPC Class Codes: A61L 27/3834 Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
• CPC Class Codes: A61M 1/02 Blood transfusion apparatus blood infusion by syringes A61M5/14
• CPC Class Codes: A61M 2202/0462 Placental blood, umbilical cord blood
• CPC Class Codes: A61M 2210/1458 Placenta
• CPC Class Codes: A61M 2210/1466 Umbilical cord
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid
• CPC Class Codes: A61P 7/06 Antianaemics
• CPC Class Codes: C12N 2509/00 Methods for the dissociation of cells, e.g. specific use of enzymes
• CPC Class Codes: C12N 2513/00 3D culture
• CPC Class Codes: C12N 2533/90 Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0605 Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696
• CPC Class Codes: C12N 5/0663 Bone marrow mesenchymal stem cells (BM-MSC)
• CPC Class Codes: C12N 5/0678 Stem cells; Progenitor cells; Precursor cells

Abstract

A method of manufacturing a tissue matrix for implantation into a patient is disclosed. The method sets forth collecting embryonic stem cells from a placenta which has been treated to remove residual cord blood and seeding the collected stem cells onto or into a tissue matrix. The seeded tissue matrix is then implanted on or into a patient. The seeded tissue matrix made by the method of the present invention is also disclosed.

Claims

1. A method of treating an individual in need of repair of traumatic injury of the skin comprising administering to the individual isolated placental stem cells, wherein said isolated placental stem cells have been seeded onto a decellularized or synthetic tissue matrix, wherein said placental stem cells are obtained from a mammalian placenta that has been drained of cord blood, and wherein said placental stem cells

(a) express antigens recognized by SH2, SH3 and SH4 antibodies;

(b) do not express MHC Class 2 antigens;

(c) are CD34⁻

, SSEA3⁻

, SSEA4⁻

, and OCT-4⁺;

(d) are SSEA3⁻

, SSEA4⁻

, OCT-4⁺ and ABC-p⁺;

or

(e) are CD10⁺, CD29⁺, CD34⁻

, CD44⁺, CD45⁻

, CD54⁺, CD90⁺, SH2⁺, SH3⁺, SH4⁺, SSEA3⁻

, SSEA4⁻

, OCT-4⁺ and ABC-p⁺.

2. The method of claim 1, wherein said placental stem cells express antigens recognized by SH2, SH3 and SH4 antibodies.

3. The method of claim 1, wherein said placental stem cells do not express MHC Class 2 antigens.

4. The method of claim 1, wherein said placental stem cells are CD34⁻

, SSEA3⁻

, SSEA4⁻

, and OCT-4⁺.

5. The method of claim 1, wherein said placental stem cells are SSEA3⁻

, SSEA4⁻

, OCT-4⁺ and ABC-p⁺.

6. The method of claim 1, wherein said placental stem cells are CD10⁺, CD29⁺, CD34⁻

, CD44⁺, CD45⁻

, CD54⁺, CD90⁺, SH2⁺, SH3⁺, SH4⁺, SSEA3⁻

, SSEA4⁻

, OCT-4⁺, and ABC-p⁺.