Silicon and germanium dyes for use in genetic identity

• US 9,139,868 B2
• Filed: 03/14/2014
• Issued: 09/22/2015
• Est. Priority Date: 03/15/2013
• Status: Active Grant

• Current Assignee: Promega Corporation
• Original Assignee: Promega Corporation
• Inventors: Zhou, Wenhui, Meisenheimer, Poncho, Zhou, Min
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US14/210,818
• Publication Number:
  US 20140272990A1
• Time in Patent Office: 557 Days
• Field of Search: 435/6.1, 536/26.6, 422/430, 549/16, 549/24, 549/214, 549/223
• US Class Current: 1/1
• CPC Class Codes: C09B 11/24 Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
• CPC Class Codes: C09B 11/28 Pyronines ; Xanthon, thioxanthon, selenoxanthan, telluroxanthon dyes
• CPC Class Codes: C09B 69/008 Dyes containing a substituent, which contains a silicium atom
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 33/582 with fluorescent label

Abstract

Si- and Ge-based dyes and methods of using same.

Claims

1. A method of detecting the presence of a nucleic acid polymer in a sample comprising:

a) contacting a sample suspected of containing a nucleic acid polymer with a composition comprising a conjugate comprising a compound of Formula (I) and an oligonucleotide; and

b) detecting the presence or amount of the compound in the sample;

wherein the compound of formula (I) is a component in an ET cassette; and

wherein the compound of formula (I) is as shown below;

2. The method of claim 1, wherein more than one nucleic acid polymer is detected in a single reaction.

3. A method of detecting the presence of a nucleic acid polymer in a sample comprising:

a) contacting a sample suspected of containing a nucleic acid polymer with a composition comprising a conjugate comprising a compound of Formula (II) and an oligonucleotide; and

b) detecting the presence or amount of the compound in the sample;

wherein the compound of formula (II) is a component in an ET cassette; and

wherein the compound of formula (II) is as shown below;

4. The method of claim 3, wherein more than one nucleic acid polymer is detected in a single reaction.

5. A compound selected from the group consisting of:

6. A kit comprising a compound according to formula (I) or (II) in an ET cassette, at least one locus-specific primer, and instructions for use;

wherein the compound of formula (I) is