Device and method for in vivo noninvasive magnetic manipulation of circulating objects in bioflows
First Claim
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1. A device for the manipulation and detection of a magnetic target object within a moving biofluid of a living organism, comprising:
- an in vivo flow cytometer for detecting the magnetic target object within an area of interest; and
at least one magnet for producing a magnetic field that manipulates the magnetic target object within the area of interest wherein the in vivo flow cytometer is an in vivo photoacoustic flow cytometer comprising a laser fiber for delivering at least one laser pulse to the area of interest, wherein the laser fiber comprises a focusing tip to transmit the at least one laser pulse to the area of interest; and
wherein the at least one magnet comprises a magnetic material forming a channel, wherein the laser fiber passes through the channel and the focusing tip is situated at a laser pulse location that is essentially coincident to a corresponding magnetic field location.
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Abstract
A device and methods for the non-invasive manipulation and detection of target objects such as cells, pathogens, microparticles, and nanoparticles in vivo using an external magnetic field are described. In one aspect, a device and method for capturing and detecting intrinsically magnetic target objects or target objects labeled with at least one magnetic particle within the area of interest using an in vivo flow cytometer are described.
83 Citations
50 Claims
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1. A device for the manipulation and detection of a magnetic target object within a moving biofluid of a living organism, comprising:
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an in vivo flow cytometer for detecting the magnetic target object within an area of interest; and at least one magnet for producing a magnetic field that manipulates the magnetic target object within the area of interest wherein the in vivo flow cytometer is an in vivo photoacoustic flow cytometer comprising a laser fiber for delivering at least one laser pulse to the area of interest, wherein the laser fiber comprises a focusing tip to transmit the at least one laser pulse to the area of interest; and wherein the at least one magnet comprises a magnetic material forming a channel, wherein the laser fiber passes through the channel and the focusing tip is situated at a laser pulse location that is essentially coincident to a corresponding magnetic field location. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method of manipulating and detecting magnetic target objects within a moving biofluid of a living organism, comprising:
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situating at least one magnet to manipulate the magnetic target objects within a magnetic field produced by the at least one magnet in an area of interest; and detecting the magnetic target objects using an in vivo flow cytometer wherein the in vivo flow cytometer is an in vivo photoacoustic flow cytometer comprising a laser fiber for delivering at least one laser pulse to the area of interest, wherein the laser fiber comprises a focusing tip to transmit the at least one laser pulse to the area of interest; and wherein the at least one magnet comprises a magnetic material forming a channel, wherein the laser fiber passes through the channel and the focusing tip is situated at a laser pulse location that is essentially coincident to a corresponding magnetic field location. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
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43. A method of detecting the presence of target cells within a biofluid of a living organism moving through a circulatory vessel, comprising:
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injecting magnetic nanoparticles conjugated with first targeting moieties into the circulatory vessel to produce magnetic target cells comprising the magnetic particles attached to the target cells; injecting photoacoustic contrast agents conjugated with second targeting moieties different than the first targeting moieties into the circulatory vessel producing magnetic target cells labeled with the photoacoustic contrast agents; situating at least one magnet to manipulate the magnetic target cells within a magnetic field produced in an area of interest by the at least one magnet; detecting the magnetic target cells in the area of interest using an in vivo photoacoustic flow cytometer; and
,subjecting the magnetic target cells to an additional process chosen from removal for biochemical or genetic analysis;
non-invasive eradication using high-energy pulses chosen from laser pulses, microwave pulses, or ultrasound pulses;
magnetic purging;
mechanical removal;
needle extraction;
or any combination thereof. - View Dependent Claims (44, 45, 46, 47, 48, 49, 50)
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Specification