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Process for culturing adherent cells

  • US 9,145,543 B2
  • Filed: 12/22/2010
  • Issued: 09/29/2015
  • Est. Priority Date: 12/23/2009
  • Status: Active Grant
First Claim
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1. A process for producing adherent cells comprising:

  • a) introducing a suspension of adherent cells into a culture vessel containing microcarriers in a culture medium, wherein the culture vessel is in direct physical contact with the culture medium;

    b) amplifying the cells by performing a plurality of cell passages in the same culture vessel, wherein each cell passage subsequent to the first cell passage is carried out;

    i) by retaining in the culture vessel all or part of the cells produced during the previous cell passage after having subjected the cells to enzyme treatment in the culture vessel to detach the cells from microcarriers without removing the all or part of the cells produced during the previous cell passage from the culture vessel, andii) by introducing culture medium and an increasing amount of microcarriers into the culture vessel; and

    c) harvesting the cells produced during the last cell passage, optionally after having subjected the cells to enzyme treatment to detach cells from microcarriers.

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