Process for preparing concentrated polyunsaturated fatty acid oil

• US 9,150,817 B2
• Filed: 04/13/2007
• Issued: 10/06/2015
• Est. Priority Date: 04/13/2006
• Status: Active Grant

• Current Assignee: Nippon Suisan Kaisha Limited
• Original Assignee: Nippon Suisan Kaisha Limited
• Inventors: Furihata, Kiyomi, Kawahara, Hiroyuki, Doisaki, Nobushige
• Primary Examiner(s): Chen, Shin Lin
• Application Number:
  US12/296,786
• Publication Number:
  US 20090176284A1
• Time in Patent Office: 3,098 Days
• Field of Search: 435/134, 435/135, 435/271, 530/350
• US Class Current: 1/1
• CPC Class Codes: C07K 14/195 from bacteria
• CPC Class Codes: C07K 14/21 from Pseudomonadaceae (F)
• CPC Class Codes: C11C 3/00 Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom sulfonated fats or oils C07C309/62; factice C08H; drying oils C09F
• CPC Class Codes: C11C 3/003 by esterification of fatty acids with alcohols C11C3/02 takes precedence
• CPC Class Codes: C11C 3/04 by esterification of fats or fatty oils
• CPC Class Codes: C12P 7/64 Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
• CPC Class Codes: C12P 7/6427 Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
• CPC Class Codes: C12P 7/6436 Fatty acid esters
• CPC Class Codes: C12P 7/6445 Glycerides
• CPC Class Codes: C12P 7/6454 by esterification
• CPC Class Codes: C12P 7/6472 containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Abstract

The present invention provides a process for concentrated PUFA oil, characterized in that alcoholysis reaction using lipase is carried out in the presence of a small amount of water and at least one compound as an additive selected from magnesium oxide, magnesium hydroxide, calcium oxide and calcium hydroxide, and then separation is conducted to obtain a glyceride fraction.

Claims

1. A process for preparing concentrated polyunsaturated fatty acid oil, which comprises:

subjecting oil and fat that contain polyunsaturated fatty acid as a fatty acid composing the oil and fat to an alcoholysis reaction using lipase in the presence of alcohol, added water and at least one compound, as a reaction additive, selected from magnesium oxide, magnesium hydroxide, calcium oxide and calcium hydroxide; and

separating a glyceride fraction that contains concentrated polyunsaturated fatty acid,

wherein the amount of the reaction additive is 0.05% to 5% (w/w) relative to the oil and fat containing polyunsaturated fatty acid, and the amount of the added water is 5% to 20% (v/v) relative to the alcohol,

wherein the polyunsaturated fatty acid is eicosapentaenoic acid, docosahexaenoic acid, arachidonic acid, or a combination thereof, and

wherein the lipase is selected from the lipases obtained from the microorganisms of Alcaligenes sp., Thermomyces lanuginosus, Burkholderia cepacia, and Pseudomonas fluorescens.

2. The process according to claim 1, wherein the reaction additive is magnesium oxide.

3. The process according to claim 2, wherein the alcoholysis reaction is ethanolysis.

4. The process according to claim 3, wherein the polyunsaturated fatty acid is eicosapentaenoic acid, docosahexaenoic acid, or a combination thereof.

5. The process according to claim 4, wherein the oil and fat containing polyunsaturated fatty acid is fish oil.

6. The process according to claim 1, wherein the alcoholysis reaction is ethanolysis.

7. The process according to claim 6, wherein the polyunsaturated fatty acid is eicosapentaenoic acid, docosahexaenoic acid, or a combination thereof.

8. The process according to claim 6, wherein the oil and fat containing polyunsaturated fatty acid is fish oil.

9. The process according to claim 1, wherein the polyunsaturated fatty acid is eicosapentaenoic acid, docosahexaenoic acid, or a combination thereof.

10. The process according to claim 1, wherein the oil and fat containing polyunsaturated fatty acid is fish oil.