High numerical aperture telemicroscopy apparatus

• US 9,154,594 B2
• Filed: 06/11/2014
• Issued: 10/06/2015
• Est. Priority Date: 01/02/2008
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Fletcher, Daniel, Hansen, Wendy, Switz, Neil, Breslauer, David N., Douglas, Erik, Maamari, Robi, Dill, Jesse, Lam, Wilbur
• Primary Examiner(s): WONG, ALLEN C
• Application Number:
  US14/301,506
• Publication Number:
  US 20150005032A1
• Time in Patent Office: 482 Days
• Field of Search: 348/79
• US Class Current: 1/1
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G02B 21/0008 Microscopes having a simple construction, e.g. portable microscopes
• CPC Class Codes: G02B 21/0092 Polarisation microscopes
• CPC Class Codes: G02B 21/02 Objectives
• CPC Class Codes: G02B 21/025 with variable magnification variable magnification G02B15/00
• CPC Class Codes: G02B 21/06 Means for illuminating specimens
• CPC Class Codes: G02B 21/16 adapted for ultraviolet illumination ; Fluorescence microscopes G02B21/0076 takes precedence
• CPC Class Codes: G02B 21/26 Stages; Adjusting means therefor
• CPC Class Codes: G02B 21/36 arranged for photographic purposes or projection purposes G02B21/18 takes precedenceor digital imaging or video purposes including associated control and data processing arrangements image data processing per se G06T
• CPC Class Codes: G02B 21/361 Optical details, e.g. image relay to the camera or image sensor G02B21/364 takes precedence; illumination details G02B21/06 and subgroups
• CPC Class Codes: G02B 21/365 Control or image processing arrangements for digital or video microscopes G02B21/361, G02B21/362 take precedence
• CPC Class Codes: H04M 1/0264 for a camera module assembly
• CPC Class Codes: H04N 5/44 Receiver circuitry for the reception of television signals according to analogue transmission standards H04N5/14 takes precedence

Abstract

An imaging system consisting of a cell-phone with camera as the detection part of an optical train which includes other components. Optionally, an illumination system to create controlled contrast in the sample. Uses include but are not limited to disease diagnosis, symptom analysis, and post-procedure monitoring, and other applications to humans, animals, and plants.

Claims

1. An apparatus for making a wireless communications device into a medical imaging system, the apparatus comprising:

a housing removably attachable to a mobile phone having a built-in camera with a camera lens and image sensor; and

a microscope lens system having high numerical aperture optics mounted to the housing and comprising a one or more lenses configured to be aligned with said camera lens when the housing is attached to the mobile phone and an eyepiece lens configured to be positioned between the microscope lens and the camera lens and to couple the high numerical aperture optics of the microscope lens system with the camera lens so that the microscope lens and the camera lens work in combination;

wherein said microscope lens system has a collection numerical aperture (NA) of at least approximately 0.01 and a magnification (M) of at least about 1.

2. The apparatus of claim 1, wherein the housing is a hand-held housing configured to hold the mobile phone.

3. The apparatus of claim 1, wherein M≧

4.

4. The apparatus of claim 1, further comprising a snap configured to hold the microscope lens system aligned with the camera lens.

5. The apparatus of claim 1, wherein the microscope lens system is telecentric on an object side, on an image side, or on both sides.

6. The apparatus of claim 1, further comprising an illumination source.

7. The apparatus of claim 6, wherein the illumination source is a fluorescence excitation source.

8. The apparatus of claim 1, further comprising a contrast selected from transillumination brightfield, darkfield, phase contrast, differential interference contrast, polarization contrast, Hoffman modulation contrast, fluorescence, Schlieren, and oblique transillumination.

9. The apparatus of claim 1, further comprising an illumination source associated with the microscope lens system.

10. The apparatus of claim 1, wherein said microscope lens system in combination with said camera lens has a collection numerical aperture (NA) of at least approximately 0.01 and a magnification (M) of at least about 1.

11. The apparatus of claim 1, wherein the microscope lens system has a collection numerical aperture (NA)>

0.1.

12. A method for making a mobile phone into a medical imaging system, the method comprising:

removably connecting a housing to a mobile phone having a built-in camera with a camera lens, and image sensor, to form a telemicroscopy apparatus, wherein said housing holds a microscope lens system, the microscope lens system having high numerical aperture optics comprising one or more microscope lenses and an eyepiece lens, in alignment with the camera lens of the camera so that the eyepiece lens of the microscope lens system is positioned between the one or more microscope lenses and the camera lens so that the high numerical aperture optics of the microscope lens system is coupled with the camera lens, so that the microscope lens and the camera lens work in combination; and

imaging a sample at a first location using the telemicroscopy apparatus wherein said microscope lens system has a collection numerical aperture (NA) of at least approximately 0.01 and a magnification (M) of at least about 1.

13. The method of claim 12, further comprising transmitting the sample image and information derived therefrom from the mobile phone to a second location.

14. The method of claim 12, further comprising providing, using software on the mobile phone, assistance to a user in acquiring images.

15. The method of claim 12, further comprising extracting information about the images on the mobile phone.

16. The method of claim 12, further comprising analyzing received images and information at the second location.

17. The method of claim 12, wherein the sample is a blood sample.

18. The method of claim 12, wherein the sample is region of a human patient.

19. The method of claim 12, wherein the sample is a region of a patient'"'"'s skin.

20. The method of claim 12, wherein said microscope lens system in combination with said camera lens has a collection numerical aperture (NA) of at least approximately 0.01 and a magnification (M) of at least about 1.

21. A method for making a mobile phone into a medical imaging system, the method comprising:

removably connecting a housing to a mobile phone having a built-in camera with a camera lens, and image sensor, to form a telemicroscopy apparatus, wherein said housing holds a microscope lens system, the microscope lens system having high numerical aperture optics comprising one or more microscope lenses and an eyepiece lens, in alignment with the camera lens of the camera so that the eyepiece lens of the microscope lens system is positioned between the one or more microscope lenses and the camera lens so that the high numerical aperture optics of the microscope lens system is coupled with the camera lens, so that the microscope lens and the camera lens work in combination;

providing, using software on the mobile phone, assistance to a user in acquiring images; and

imaging a sample at a first location using the telemicroscopy apparatus wherein said microscope lens system has a collection numerical aperture (NA) of at least approximately 0.01 and a magnification (M) of at least about 1.