Defined cell culturing surfaces and methods of use
First Claim
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1. A cell culturing substrate comprising:
- a cell culture surface having a film attached thereto, wherein said film comprises one or more plasma polymerized monomers; and
a coating on said film-coated surface comprising one or more extracellular matrix proteins comprising fibronectin, laminin or a combination thereof.
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Abstract
In one aspect, there is provided a cell culturing substrate including:
- a cell culture surface having a film attached thereto, wherein the film includes one or more plasma polymerized monomers; and a coating on the film-coated surface, the coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, where the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.
7 Citations
34 Claims
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1. A cell culturing substrate comprising:
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a cell culture surface having a film attached thereto, wherein said film comprises one or more plasma polymerized monomers; and a coating on said film-coated surface comprising one or more extracellular matrix proteins comprising fibronectin, laminin or a combination thereof. - View Dependent Claims (2, 3, 4, 5, 9, 10, 12, 13, 14)
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6. A cell culturing substrate comprising:
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(i) a cell culture surface having a film attached thereto, wherein said film comprises one or more plasma polymerized monomers; and (ii) a coating on said film-coated surface comprising; (a) one or more extracellular matrix proteins, and (b) a component selected from the group consisting of entactin, heparan sulfate proteoglycans (HSPG), growth factors and combinations thereof. - View Dependent Claims (7, 8, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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11. A cell culturing substrate comprising:
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a cell culture surface having a film attached thereto, wherein said film comprises one or more plasma polymerized monomers; and a coating on said film-coated surface comprising one or more extracellular matrix proteins, wherein the cell culture surface comprises hollow fiber tubes or micro carrier beads. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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Specification