Interlocking cap and receptacle for automated processes

• US 9,162,228 B2
• Filed: 03/13/2014
• Issued: 10/20/2015
• Est. Priority Date: 03/14/2013
• Status: Active Grant

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Inventors: Knight, Byron J.
• Primary Examiner(s): Hyun, Paul
• Application Number:
  US14/210,042
• Publication Number:
  US 20140263153A1
• Time in Patent Office: 586 Days
• Field of Search: 422/550, 422/568
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/02 Adapting objects or devices to another
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/026 Fluid interfacing between devices or objects, e.g. connectors, inlet details
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 2200/142 Preventing evaporation
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/042 Caps; Plugs
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/047 Additional chamber, reservoir
• CPC Class Codes: B01L 2300/048 enabling gas exchange, e.g. vents
• CPC Class Codes: B01L 2300/0609 Holders integrated in container to position an object
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0832 cylindrical, tube shaped
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2300/0851 Bottom walls
• CPC Class Codes: B01L 2300/0858 Side walls
• CPC Class Codes: B01L 2300/12 Specific details about materials
• CPC Class Codes: B01L 2400/0406 : capillary forces
• CPC Class Codes: B01L 3/502 : with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/5082 : Test tubes per se
• CPC Class Codes: B01L 3/50825 : Closing or opening means, corks, bungs closures for containers B65D; means for removing stoppers B67B7/02
• CPC Class Codes: B01L 3/5085 : for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/50853 : with covers or lids
• CPC Class Codes: B01L 3/50855 : using modular assemblies of strips or of individual wells
• CPC Class Codes: B01L 3/523 : with means for closing or opening
• CPC Class Codes: B01L 3/527 : for a plurality of reagents
• CPC Class Codes: B65B 69/00 : Unpacking of articles or materials, not otherwise provided for
• CPC Class Codes: B65D 1/0207 : characterised by material, e.g. composition, physical features
• CPC Class Codes: B65D 1/34 : Trays or like shallow containers B65D81/3813 takes precedence
• CPC Class Codes: B65D 1/36 : with moulded compartments or partitions
• CPC Class Codes: B65D 17/50 : Non-integral frangible members applied to, or inserted in, preformed openings, e.g. tearable strips or plastic plugs B65D53/08 takes precedence
• CPC Class Codes: B65D 2539/003 : provided with sealing flanges or ribs
• CPC Class Codes: B65D 39/0017 : Injection-molded plastic closures for "Champagne"- or "Sekt"-type bottles
• CPC Class Codes: B65D 39/0029 : Plastic closures other than those covered by groups B65D39/0011 - B65D39/0023
• CPC Class Codes: C12Q 1/686 : Polymerase chain reaction [PCR]
• CPC Class Codes: G01N 2035/00287 : movable lid/cover for sample or reaction tubes
• CPC Class Codes: G01N 30/6091 : Cartridges
• CPC Class Codes: G01N 35/0099 : comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: G01N 35/026 : having blocks or racks of reaction cells or cuvettes
• CPC Class Codes: G01N 35/10 : Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices G01N35/0099 takes precedence
• CPC Class Codes: G01N 35/1002 : Reagent dispensers
• CPC Class Codes: G01N 35/1079 : with means for piercing stoppers or septums
• CPC Class Codes: Y10T 436/11 : Automated chemical analysis

Abstract

System, apparatuses, and methods for performing automated reagent-based analysis are provided. Also provided are methods for automated attachment of a cap to a reaction receptacle, and automated removal of a cap from a capped reaction receptacle.

Claims

1. A cap securable to a receptacle and comprising:

a lower portion for inserting into a receptacle opening;

an upper portion having an opening formed therein, the opening defining an open end that is configured for engagement by a receptacle transport mechanism;

a plurality of locking arms extending toward the lower portion of the cap and configured for engaging a portion of a receptacle to secure the cap to the receptacle when the lower portion is inserted into the receptacle opening, each of the locking arms extending axially from an annular flange extending radially with respect to a centerline of the cap and defining a gap between an outer surface of the lower portion and an inner surface of each locking arm, wherein the gap is sized to receive a lip surrounding the receptacle opening when the lower portion is inserted into the receptacle opening and the locking arms securely engage the lip surrounding the receptacle opening; and

a plurality of longitudinally extending linear ribs disposed on an inner surface of the cap, the inner surface of the cap being defined by the opening formed in the upper portion, wherein each of the linear ribs has associated therewith at least one of;

(i) an enlarged portion proximate a distal end thereof, and (ii) a recess disposed on an outer surface of the upper portion, wherein the recess corresponds in length with the associated rib and is disposed on the outer surface opposite the associated rib.

2. The cap of claim 1, wherein the lower portion of the cap has an outer surface defining one or more annular ribs configured to provide a sealing engagement between the outer surface and an inner surface of the receptacle opening.

3. The cap of claim 1, wherein a proximal end of each of the linear ribs is beveled.

4. The cap of claim 1, wherein each of the linear ribs includes the enlarged portion, and wherein each enlarged portion has a greater radius than the remainder of the associated rib.

5. The cap of claim 1, wherein the opening formed in the upper portion of the cap defines an upper, generally cylindrical portion and a lower, inwardly tapered portion.

6. The cap of claim 4, wherein the opening formed in the upper portion defines an upper, generally cylindrical portion and a lower, inwardly tapered portion, and wherein the enlarged portion of each of the linear ribs traverses a transition between the cylindrical portion of the opening and the inwardly tapered portion of the opening.

7. The cap of claim 1, wherein each of the linear ribs has associated therewith the recess disposed on the outer surface of the upper portion, and wherein each of the linear ribs and the associated recess are configured to flex radially outwardly to accommodate the shape of the receptacle transport mechanism inserted into the open end of the cap.

8. The cap of claim 1, wherein each of the linear ribs includes an inwardly tapered portion proximate the distal end thereof.

9. The cap of claim 1, further comprising an opening formed in the lower portion and a bottom wall separating the opening formed in the upper portion from the opening formed in the lower portion.

10. The cap of claim 9, wherein the bottom wall includes score lines formed therein to facilitate piercing of the bottom wall by a mechanism configured to dispense reagents.

11. The cap of claim 1, wherein the locking arms facilitate a snap fit attachment for securely engaging a lip surrounding the receptacle opening.

12. The cap of claim 1, wherein at least a portion of the cap is comprised of an opaque material.

13. The cap of claim 12, wherein the opaque material comprises a material that exhibits no autofluorescence.

14. The cap of claim 1, wherein the cap is a unitary plastic structure.

15. The cap of claim 1, wherein the receptacle transport mechanism is an automated pipettor or a pick-and-place robot, and wherein a tubular probe of the automated pipettor or the pick-and-place robot is configured for frictional engagement with the open end of the cap.

16. A combination of a receptacle and the cap of claim 1, each of the receptacle and the cap being carried within a well of a tray, wherein the receptacle is configured for engagement by the cap and comprises:

a generally cylindrical portion having an opening formed therein and configured to receive the lower portion of the cap so as to provide a sealing engagement between an outer surface of the lower portion of the cap and an inner surface of the opening formed in the cylindrical portion of the receptacle; and

a radially projecting lip circumscribing the opening of the receptacle, the lip being configured for interlocking engagement with the locking arms of the cap.

17. The combination of claim 16, wherein the receptacle further comprises a conical portion depending from the cylindrical portion, and wherein the opening of the receptacle continues into the conical portion.

18. The combination of claim 17, wherein the receptacle further comprises an annular ring extending outwardly from an outer surface thereof.

19. The combination of claim 16, wherein the receptacle further comprises a plurality of longitudinally oriented grooves formed in an inner surface of the opening.

20. The combination of claim 16 wherein each of the cap and the receptacle is a unitary plastic structure.

21. An assembly comprising a receptacle and a separate cap in interlocking engagement with each other,

wherein the receptacle comprises:

a generally cylindrical portion having an opening formed therein; and

a radially projecting lip circumscribing the opening; and

wherein the cap comprises;

a lower portion inserted into the opening of the receptacle and configured to provide a sealing engagement between an outer surface of the lower portion of the cap and an inner surface of the opening of the receptacle;

an upper portion having an opening formed therein, the opening defining an open end that is configured for engagement by a receptacle transport mechanism;

a plurality of locking arms extending toward the lower portion of the cap and engaging the lip of the receptacle in an interlocking relationship, thereby securing the cap to the receptacle, wherein each of the locking arms extends axially from an annular flange extending radially with respect to a centerline of the cap and defining a gap between an outer surface of the lower portion and an inner surface of the locking arm, and wherein the lip of the receptacle extends into the gap; and

a plurality of longitudinally extending linear ribs disposed on an inner surface of the cap, the inner surface of the cap being defined by the opening formed in the upper portion, wherein each of the linear ribs has associated therewith at least one of;

(i) an enlarged portion proximate a distal end thereof, and (ii) a recess disposed on an outer surface of the upper portion, wherein the recess corresponds in length with the associated rib and is disposed on the outer surface opposite the associated rib.

22. The assembly of claim 21, wherein the lower portion of the cap has an outer surface defining one or more annular ribs that provide a sealing engagement between the outer surface of the cap and the inner surface of the opening of the receptacle.

23. The assembly of claim 21, wherein each of the linear ribs includes an inwardly tapered portion proximate the distal end thereof.

24. The assembly of claim 21, wherein the cap further comprises an opening formed in the lower portion and a bottom wall separating the opening formed in the upper portion from the opening formed in the lower portion.

25. The assembly of claim 24, wherein the bottom wall includes score lines formed therein to facilitate piercing of the bottom wall by a mechanism configured to dispense reagents to a test sample contained within the receptacle.

26. The assembly of claim 21, wherein the interlocking relationship is a snap fit attachment of the cap to the receptacle.

27. The assembly of claim 21, wherein the receptacle further comprises a conical portion depending from the cylindrical portion, wherein the opening continues into the conical portion.

28. The assembly of claim 21, wherein the receptacle further comprises a plurality of longitudinally oriented grooves formed on an inner surface of the opening of the receptacle.

29. The assembly of claim 21, wherein each of the cap and the receptacle is a unitary plastic structure.

30. The assembly of claim 21, wherein the receptacle transport mechanism is an automated pipettor or a pick-and-place robot, and wherein a tubular probe of the automated pipettor or the pick-and-place robot is configured for frictional engagement with the open end of the cap.