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Device and method for the regeneration of biosensors

  • US 9,162,259 B2
  • Filed: 09/12/2008
  • Issued: 10/20/2015
  • Est. Priority Date: 09/18/2007
  • Status: Expired due to Fees
First Claim
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1. A fluorescent excitation biosensor having a light source that is positioned on the backside of a surface, comprising an immobilized and biologically active material that is applied to the surface of a carrier and is suited to interact with a substance to be analyzed, wherein the surface of the carrier is an optically transparent semiconductor in form of a gallium nitride-, a zinc oxide- or a diamond coating, or a composition including these materials, epitaxially grown on a sapphire substance;

  • the biosensor further including a regenerating device comprising;

    at least two solution containers each containing different solutions, wherein at least one solution is a molecular-removal solution and at least one solution is a biologically active material; and

    an automated microfluid system integrated into the biosensor comprising;

    at least one injector through which, via a pump, at least one of the solutions is applied onto the surface of the carrier for rinsing the biologically active material;

    further comprising a valve arrangement, though which the solutions are applied successively by the injector and the pump to the surface of the carrier; and

    wherein the reagents and/or the carrier are temperature-controlled; and

    /orwherein a chamber with the carrier can be subjected to ultrasound.

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