System and method for sensing NO

• US 9,164,080 B2
• Filed: 06/11/2012
• Issued: 10/20/2015
• Est. Priority Date: 06/11/2012
• Status: Active Grant

• Current Assignee: Ohio State Innovation Foundation (Ohio State University)
• Original Assignee: Ohio State Innovation Foundation (Ohio State University)
• Inventors: Dutta, Prabir K., Mondal, Suvra P.
• Primary Examiner(s): Caputo, Lisa
• Assistant Examiner(s): ROY, PUNAM P
• Application Number:
  US13/493,846
• Publication Number:
  US 20130327122A1
• Time in Patent Office: 1,226 Days
• US Class Current: 1/1
• CPC Class Codes: A61B 5/082 Evaluation by breath analysis, e.g. determination of the chemical composition of exhaled breath A61B5/083, A61B5/091 take precedence
• CPC Class Codes: G01N 2033/4975 other than oxygen, carbon dioxide or alcohol, e.g. organic vapours
• CPC Class Codes: G01N 33/0037 for NOx
• CPC Class Codes: G01N 33/497 of gaseous biological material, e.g. breath
• CPC Class Codes: Y02A 50/20 Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Abstract

An NO sensing system includes an inlet for receiving an original sample, a humidifier, fluidly communicating with the inlet, and a first sensor. The original sample is fluidly transmitted through the humidifier and exits the humidifier as a humidified sample having a humidity above a predetermined level. The first sensor generates a potential difference in response to presence of NO in the humidified sample. The potential difference is indicative of a level of NO within the original sample.

Claims

1. An NO sensing system for measuring NO in a breath sample, the system comprising:

an inlet configured to receive the breath sample as it is exhaled by a subject;

a humidifier fluidly communicating with the inlet and configured to humidify the breath sample as it transmits the breath sample through the humidifier to a humidifier outlet, such that the breath sample exits the humidifier outlet as a humidified sample having a humidity above a predetermined level; and

a sensing element comprising a plurality of sensors electrically coupled together, each of the plurality of sensors configured to generate a potential difference in response to presence of NO in the humidified sample;

wherein the plurality of sensors comprises an effective number of sensors coupled together such that a combined potential difference of the plurality of sensors can indicate a level of NO less than about 100 ppb in the breath sample.

2. The NO sensing system as set forth in claim 1, wherein the humidified sample has a humidity of about 100%.

3. The NO sensing system as set forth in claim 1, wherein the combined potential difference of the plurality of sensors includes a sum of the potential differences of each of the plurality of sensors.

4. The NO sensing system as set forth in claim 3, wherein the plurality of sensors comprises at least 15 sensors.

5. The NO sensing system as set forth in claim 4, wherein the sum of the potential differences of the at least 15 sensors is capable of indicating a level of NO down to about 10 ppb in the breath sample.

6. The NO sensing system as set forth in claim 1, wherein the plurality of sensors are electrically connected together in series.

7. The NO sensing system as set forth in claim 6, wherein each of the plurality of sensors includes a sensing electrode and a reference electrode,

wherein the sensing element further comprises:

a first electrical lead electrically coupled to the sensing electrode of a first sensor of the plurality of sensors electrically connected together in series;

a second electrical lead electrically coupled to the reference electrode of a last sensor of the plurality of sensors electrically connected together in series; and

wherein the combined potential difference of the plurality of sensors is measurable between the first and second electrical leads.

8. The NO sensing system as set forth in claim 1, wherein the system further comprises a heater warming the plurality of sensors.

9. The NO sensing system as set forth in claim 1, wherein the humidifier includes a humidifier inlet configured to direct the breath sample into a liquid fluid;

such that the breath sample passes through the liquid fluid and exits the liquid fluid as the humidified sample.

10. The NO sensing system as set forth in claim 9, wherein passing through the liquid fluid comprises bubbling through the liquid fluid.

11. The NO sensing system as set forth in claim 1, wherein the plurality of sensors comprises at least 8 sensors.

12. A method of sensing NO in a breath sample, the method comprising:

receiving a breath sample from a subject;

humidifying the breath sample into a humidified sample having a humidity above a predetermined level; and

contacting a sensing element with the humidified sample, wherein the sensing element comprises a plurality of sensors electrically coupled together, thereby generating a combined potential difference of the plurality of sensors in response to presence of NO in the humidified sample;

wherein the plurality of sensors comprises an effective number of sensors coupled together such that the combined potential difference of the plurality of sensors can indicate a level of NO less than about 100 ppb in the breath sample.

13. The method of sensing NO as set forth in claim 12, further comprising determining a level of NO within the breath sample based on the combined potential difference generated by the plurality of sensors.

14. The method of sensing NO as set forth in claim 12, further comprising warming the sensing element.

15. The method of sensing NO as set forth in claim 12, wherein humidifying the breath sample comprises passing the breath sample through a liquid fluid to result in the humidified sample.

16. The method of sensing NO as set forth in claim 12, wherein passing the breath sample through the liquid fluid comprises bubbling the original sample through the liquid fluid.

17. The method of sensing NO as set forth in claim 12, wherein the plurality of sensors are electrically connected together in series.

18. The method of sensing NO as set forth in claim 17, wherein each of the plurality of sensors includes a sensing electrode and a reference electrode,

wherein the sensing element further comprises:

a first electrical lead electrically coupled to the sensing electrode of a first sensor of the plurality of sensors electrically connected together in series;

a second electrical lead electrically coupled to the reference electrode of a last sensor of the plurality of sensors electrically connected together in series; and

wherein the combined potential difference of the plurality of sensors is measurable between the first and second electrical leads.

19. An NO sensing system for measuring NO in a breath sample, the system comprising:

an inlet for receiving the breath sample from a subject;

means for humidifying the breath sample above a predetermined level resulting in a humidified breath sample; and

a sensing element comprising a plurality of sensors electrically coupled together in series, each of the plurality of sensors configured to generate a potential difference in response to presence of NO in the humidified breath sample;

wherein the plurality of sensors comprises an effective number of sensors coupled together in series such that a combined potential difference of the plurality of sensors can indicate a level of NO less than about 100 ppb in the breath sample.

20. The NO sensing system as set forth in claim 19, further including:

a heater warming the sensor to be within a range of about 450°

C. and about 650°

C.