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Methods and systems for fast PCR heating

  • US 9,168,530 B2
  • Filed: 12/16/2011
  • Issued: 10/27/2015
  • Est. Priority Date: 12/17/2010
  • Status: Active Grant
First Claim
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1. A microplate for polymerase chain reaction (PCR), comprising:

  • a substrate comprising a metallic material for heating a sample during PCR;

    a barrier layer coated on the substrate, the barrier layer formed of a first polymeric material;

    a moulding comprising one or more wells for holding said sample during PCR sealed to the barrier layer, the one or more wells formed of a second polymeric material that is separate from the first polymeric material; and

    fingerlike electrodes as projections from the substrate with corrugated surfaces in electrical communication with the substrate, which electrodes direct electrical current through the substrate,wherein the substrate is adapted to heat a sample during PCR upon the flow of electrical current through the substrate, and wherein the substrate provides a PCR ramp rate of at least 5°

    C./second.

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