Methods and systems for fast PCR heating

• US 9,168,530 B2
• Filed: 12/16/2011
• Issued: 10/27/2015
• Est. Priority Date: 12/17/2010
• Status: Active Grant

• Current Assignee: BJS IP Ltd.
• Original Assignee: BJS IP Ltd.
• Inventors: Gunter, Ian, Lewis, Richard
• Primary Examiner(s): MUMMERT, STEPHANIE KANE
• Application Number:
  US13/329,183
• Publication Number:
  US 20120214207A1
• Time in Patent Office: 1,411 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/1811 using electromagnetic induction heating
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 7/54 using spatial temperature gradients
• CPC Class Codes: C12P 19/34 Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

A microplate for polymerase chain reaction (PCR) comprises a substrate having a metallic material for heating PCR samples, and a barrier layer disposed adjacent to the substrate. In some cases, the barrier layer is formed of a first polymeric material. The microplate includes one or more wells for containing PCR samples. The one or more wells are formed of a second polymeric material sealed to the barrier layer. In some cases, the substrate can provide a PCR ramp rate of at least about 5° C./second.

Claims

1. A microplate for polymerase chain reaction (PCR), comprising:

a substrate comprising a metallic material for heating a sample during PCR;

a barrier layer coated on the substrate, the barrier layer formed of a first polymeric material;

a moulding comprising one or more wells for holding said sample during PCR sealed to the barrier layer, the one or more wells formed of a second polymeric material that is separate from the first polymeric material; and

fingerlike electrodes as projections from the substrate with corrugated surfaces in electrical communication with the substrate, which electrodes direct electrical current through the substrate,

wherein the substrate is adapted to heat a sample during PCR upon the flow of electrical current through the substrate, and wherein the substrate provides a PCR ramp rate of at least 5°

C./second.

2. The microplate of claim 1, wherein the metallic material comprises aluminum.

3. The microplate of claim 1, wherein the substrate increases the temperature of said sample at a rate between about 1°

C./s and 35°

C./s.

4. The microplate of claim 1, wherein the metallic material has a resistivity between about 2×

10<sup>−

8</sup> ohm-m and 8×

10<sup>−

8</sup> ohm-m.

5. The microplate of claim 1, wherein the one or more wells comprise at least 24 wells.

6. The microplate of claim 1, wherein the one or more wells comprise at least 96 wells.

14. The microplate of claim 1, wherein the substrate is adapted to heat a sample during PCR upon flow of electrical current through the substrate.

17. The microplate of claim 1, wherein said first polymeric material is chemically compatible with said second polymeric material.

7. A microplate for polymerase chain reaction (PCR), comprising:

a substrate comprising a metallic material for heating a sample during PCR;

a barrier layer coated adjacent to the substrate, the barrier layer formed of a first polymeric material;

a moulding comprising one or more wells sealed to the barrier layer for containing said sample during PCR, the one or more wells formed of a second polymeric material that is separate from the first polymeric material; and

fingerlike electrodes as projections from the substrate with corrugated surfaces in electrical communication with the substrate, which electrodes direct electrical current through the substrate,

wherein the substrate is adapted to heat a sample during PCR upon the flow of electrical current through the substrate, and wherein the substrate provides well-to-well thermal uniformity of +/−

1°

C. or better without an external heating element or a Peltier heating block.

15. The microplate of claim 7, wherein the substrate is adapted to heat a sample during PCR upon flow of electrical current through the substrate.

18. The microplate of claim 7, wherein said first polymeric material is chemically compatible with said second polymeric material.

8. A microplate for polymerase chain reaction (PCR), comprising:

a substrate comprising a metallic material for heating a sample during PCR;

a barrier layer coated on the substrate, the barrier layer formed of a first polymeric material;

a moulding comprising one or more wells for containing said sample during PCR sealed to the barrier layer, the one or more wells formed of a second polymeric material that is separate from the first polymeric material; and

fingerlike electrodes as projections from the substrate with corrugated surfaces in electrical communication with the substrate, which electrodes direct electrical current through the substrate, wherein the substrate is adapted to heat a sample during PCR upon the flow of electrical current through the substrate, and wherein the substrate provides a heating efficiency sufficient for at least about 1 PCR cycle per minute, including fluorescence measurement for every cycle.

9. The microplate of claim 8, wherein the substrate comprises aluminum.

10. The microplate of claim 8, wherein the microplate has a thickness of less than 1 mm.

11. The microplate of claim 8, wherein the barrier layer has a thickness of less than 50 microns.

12. The microplate of claim 8, further comprising an infrared radiation-normalizing layer at a side of the substrate opposite the barrier layer.

13. The microplate of claim 12 wherein the infrared radiation-normalizing layer has a thickness of less than 50 microns.

16. The microplate of claim 8, wherein the substrate is adapted to heat a sample during PCR upon flow of electrical current through the substrate.

19. The microplate of claim 8, wherein said first polymeric material is chemically compatible with said second polymeric material.