Methods and systems for fast PCR heating
First Claim
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1. A microplate for polymerase chain reaction (PCR), comprising:
- a substrate comprising a metallic material for heating a sample during PCR;
a barrier layer coated on the substrate, the barrier layer formed of a first polymeric material;
a moulding comprising one or more wells for holding said sample during PCR sealed to the barrier layer, the one or more wells formed of a second polymeric material that is separate from the first polymeric material; and
fingerlike electrodes as projections from the substrate with corrugated surfaces in electrical communication with the substrate, which electrodes direct electrical current through the substrate,wherein the substrate is adapted to heat a sample during PCR upon the flow of electrical current through the substrate, and wherein the substrate provides a PCR ramp rate of at least 5°
C./second.
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Abstract
A microplate for polymerase chain reaction (PCR) comprises a substrate having a metallic material for heating PCR samples, and a barrier layer disposed adjacent to the substrate. In some cases, the barrier layer is formed of a first polymeric material. The microplate includes one or more wells for containing PCR samples. The one or more wells are formed of a second polymeric material sealed to the barrier layer. In some cases, the substrate can provide a PCR ramp rate of at least about 5° C./second.
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Citations
19 Claims
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1. A microplate for polymerase chain reaction (PCR), comprising:
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a substrate comprising a metallic material for heating a sample during PCR; a barrier layer coated on the substrate, the barrier layer formed of a first polymeric material; a moulding comprising one or more wells for holding said sample during PCR sealed to the barrier layer, the one or more wells formed of a second polymeric material that is separate from the first polymeric material; and fingerlike electrodes as projections from the substrate with corrugated surfaces in electrical communication with the substrate, which electrodes direct electrical current through the substrate, wherein the substrate is adapted to heat a sample during PCR upon the flow of electrical current through the substrate, and wherein the substrate provides a PCR ramp rate of at least 5°
C./second. - View Dependent Claims (2, 3, 4, 5, 6, 14, 17)
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7. A microplate for polymerase chain reaction (PCR), comprising:
- a substrate comprising a metallic material for heating a sample during PCR;
a barrier layer coated adjacent to the substrate, the barrier layer formed of a first polymeric material; a moulding comprising one or more wells sealed to the barrier layer for containing said sample during PCR, the one or more wells formed of a second polymeric material that is separate from the first polymeric material; and fingerlike electrodes as projections from the substrate with corrugated surfaces in electrical communication with the substrate, which electrodes direct electrical current through the substrate, wherein the substrate is adapted to heat a sample during PCR upon the flow of electrical current through the substrate, and wherein the substrate provides well-to-well thermal uniformity of +/−
1°
C. or better without an external heating element or a Peltier heating block. - View Dependent Claims (15, 18)
- a substrate comprising a metallic material for heating a sample during PCR;
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8. A microplate for polymerase chain reaction (PCR), comprising:
- a substrate comprising a metallic material for heating a sample during PCR;
a barrier layer coated on the substrate, the barrier layer formed of a first polymeric material; a moulding comprising one or more wells for containing said sample during PCR sealed to the barrier layer, the one or more wells formed of a second polymeric material that is separate from the first polymeric material; and fingerlike electrodes as projections from the substrate with corrugated surfaces in electrical communication with the substrate, which electrodes direct electrical current through the substrate, wherein the substrate is adapted to heat a sample during PCR upon the flow of electrical current through the substrate, and wherein the substrate provides a heating efficiency sufficient for at least about 1 PCR cycle per minute, including fluorescence measurement for every cycle. - View Dependent Claims (9, 10, 11, 12, 13, 16, 19)
- a substrate comprising a metallic material for heating a sample during PCR;
Specification