Ketol-acid reductoisomerase enzymes and methods of use
First Claim
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1. A polypeptide having ketol-acid reductoisomerase activity, wherein the polypeptide comprisesa. the amino acid sequence of SEQ ID NO:
- 41;
b. at least 99% identity to SEQ ID NO;
41;
c. at least 99% identity to SEQ ID NO;
41 and at least one of the following substitutions;
M301I, Y239H, Y113F, S322A, A71K, N114G, A329R, A69T, N114S, G72W, A295V, E264K, R280D, A329E, S157T, M238I, Q272T, K335M, R280H, and a combination thereof;
ord. an active fragment of any one of (a) to (c).
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Abstract
Provided herein are polypeptides having ketol-aid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.
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Citations
12 Claims
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1. A polypeptide having ketol-acid reductoisomerase activity, wherein the polypeptide comprises
a. the amino acid sequence of SEQ ID NO: - 41;
b. at least 99% identity to SEQ ID NO;
41;c. at least 99% identity to SEQ ID NO;
41 and at least one of the following substitutions;
M301I, Y239H, Y113F, S322A, A71K, N114G, A329R, A69T, N114S, G72W, A295V, E264K, R280D, A329E, S157T, M238I, Q272T, K335M, R280H, and a combination thereof;
ord. an active fragment of any one of (a) to (c). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
- 41;
Specification