×

Process for producing alpha-1,3-glucan polymer with reduced molecular weight

  • US 9,169,506 B2
  • Filed: 09/04/2014
  • Issued: 10/27/2015
  • Est. Priority Date: 09/05/2013
  • Status: Active Grant
First Claim
Patent Images

1. A process for producing poly alpha-1,3-glucan comprising:

  • (a) contacting water, sucrose, a polar organic solvent, and a bacterial glucosyltransferase enzyme in a solution, wherein the glucosyltransferase enzyme synthesizes poly alpha-1,3-glucan, wherein a poly alpha-1,3-glucan is produced having a reduced molecular weight compared to the molecular weight of the poly alpha-1,3-glucan produced in the absence of the polar organic solvent, wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 97% identical to SEQ ID NO;

    12, SEQ ID NO;

    2, SEQ ID NO;

    32, SEQ ID NO;

    4, SEQ ID NO;

    6, SEQ ID NO;

    8, SEQ ID NO;

    10, SEQ ID NO;

    14, SEQ ID NO;

    18, SEQ ID NO;

    20, SEQ ID NO;

    22, SEQ ID NO;

    24, SEQ ID NO;

    26, SEQ ID NO;

    28, or SEQ ID NO;

    30; and

    (b) optionally, isolating the poly alpha-1,3-glucan produced in step (a);

    wherein the polar organic solvent is selected from the group consisting of acetonitrile, dimethyl sulfoxide, acetone, N,N-dimethylformamide, N,N-dimethylacetamide, tetrahydrofuran, propylene carbonate, sulfolane, hexamethylphosphoramide, 1,3-dimethyl-2-imidazolidinone, nitromethane, butanone, methanol, methyl formamide, ethanol, isopropanol, 1-propanol, tert-butyl alcohol, formamide, n-butanol, ethylene glycol, 2-methoxyethanol, 1-methoxy-2-propanol, glycerol, 1,2-propanediol, and 1,3-propanetriol.

View all claims
  • 4 Assignments
Timeline View
Assignment View
    ×
    ×