Fluid associated with adult stem cells for medical, cosmetic, and veterinary use

• US 9,173,903 B2
• Filed: 08/13/2010
• Issued: 11/03/2015
• Est. Priority Date: 08/17/2009
• Status: Active Grant

• Current Assignee: Ashok K. Singh
• Original Assignee: Ashok K. Singh
• Inventors: Singh, Ashok K.
• Primary Examiner(s): Ton, Thaian N
• Application Number:
  US12/855,760
• Publication Number:
  US 20110038903A1
• Time in Patent Office: 1,908 Days
• US Class Current: 1/1
• CPC Class Codes: A61K 35/24 Mucus; Mucous glands; Bursa; Synovial fluid; Arthral fluid; Excreta; Spinal fluid saliva A61K35/38
• CPC Class Codes: A61P 13/00 Drugs for disorders of the urinary system
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system

Abstract

Apparatus and process to create, expand, and localize the population of stem cells in adult tissues by placing a foreign body either in omentum or in the subcutaneous tissue. The apparatus 1) traps the granulation fluid, cells a fluid rich in a myriad of growth factors, 2) mobilizes and concentrates free-floating stem cells in the granulation fluid, and 3) stimulates the growth of a new tissue around the apparatus, which provides another source of stem cells. Stem cells obtained by this process can be used as freshly isolated or after culturing, which can be used for wound healing, treating diseases, and regenerating organs in animals and humans. The granulation fluid could substitute the stem cells in many of their applications and offers the convenience of an injectable medicine for treating animal and human diseases.

Claims

1. A method for treating a condition associated with damaged or diseased tissue in a patient, the method comprising administering to the patient a therapeutically effective amount of fluid isolated from a granuloma induced by a foreign body implanted under the skin or in the omentum of a live mammal, wherein the fluid so obtained contains albumin, globulins and at least 2 ng/mL of vascular endothelial growth factor (VEGF), and exceeds FBS in potency for promoting the growth of HK and primary mesangial cells in culture media, and the method results in an improvement in the patient'"'"'s condition.

2. The method of claim 1, wherein the method results in the repair or regeneration of at least a portion of the damaged or diseased tissue.

3. The method of claim 1, wherein the damaged or diseased tissue comprises arthritic tissue.

4. The method of claim 1, wherein the damaged or diseased tissue comprises organ tissue.

5. The method of claim 1, wherein the damaged or diseased tissue comprises joint tissue, intervertebral disc tissue, skin, kidney tissue, liver tissue, heart tissue, spinal cord tissue, surgically wounded tissue, or a combination thereof.

6. The method of claim 5, wherein the damaged or diseased tissue comprises joint tissue.

7. The method of claim 6, wherein the joint tissue is knee joint tissue.

8. The method of claim 6, wherein the patient is a dog.

9. The method of claim 6, wherein the patient is a horse.

10. The method of claim 1, wherein the granuloma fluid is administered topically.

11. The method of claim 1, wherein the granuloma fluid is administered systemically.

12. The method of claim 11, wherein the granuloma fluid is administered by injection.

13. The method of claim 11, wherein the granuloma fluid is administered intravenously.

14. The method of claim 11, wherein the granuloma fluid is administered subcutaneously.

15. The method of claim 11, wherein the granuloma fluid is administered intraperitoneally.

16. The method of claim 1, wherein the granuloma fluid is harvested from a pig, cow, horse, sheep, dog, rat, or rabbit.

17. The method of claim 1, wherein the granuloma fluid is obtained by reconstituting a solid form of the granuloma fluid.

18. The method of claim 1, wherein the granuloma fluid is isolated by centrifugation.

19. The method of claim 1, wherein the patient is a human, horse or dog.

20. The method of claim 1, wherein the patient is a human.

21. The method of claim 1, wherein the patient is a horse.

22. The method of claim 1, wherein the patient is a dog.

23. A method for treating arthritis in a patient, the method comprising administering to the patient a therapeutically effective amount of fluid isolated from a granuloma induced by a foreign body implanted under the skin or in the omentum a live mammal, wherein the fluid so obtained contains albumin, globulins and at least 2 ng/mL of vascular endothelial growth factor (VEGF), and exceeds FBS in potency for promoting the growth of HK and primary mesangial cells in culture media, and the method results in an improvement of the arthritic condition.

24. The method of claim 23, wherein the method comprises treating an arthritic joint.

25. The method of claim 24, wherein the joint is a knee joint.

26. The method of claim 23, wherein the granuloma fluid is obtained by reconstituting a solid form of the granuloma fluid.

27. The method of claim 26, wherein the granuloma fluid is harvested from a horse.

28. The method of claim 27, wherein the patient is human.

29. The method of claim 23, wherein the granuloma fluid is isolated by centrifugation.

30. The method of claim 29, wherein the granuloma fluid is harvested from a horse.

31. The method of claim 30, wherein the patient is human.

32. The method of claim 23, wherein the patient is a human, horse or dog.

33. The method of claim 23, wherein the patient is a human.

34. The method of claim 23, wherein the patient is a horse.

35. The method of claim 23, wherein the patient is a dog.