Imprinted polymer nanoparticles

• US 9,173,943 B2
• Filed: 01/11/2010
• Issued: 11/03/2015
• Est. Priority Date: 01/12/2009
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Hoshino, Yu, Shea, Kenneth J.
• Primary Examiner(s): JONES JR., ROBERT STOCKTON
• Application Number:
  US13/144,027
• Publication Number:
  US 20120097613A1
• Time in Patent Office: 2,122 Days
• Field of Search: 525/54.1, 530/402, 530/415, 524/849, 524/850, 524/853
• US Class Current: 1/1
• CPC Class Codes: A61K 47/32 Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers , poly(meth)acrylates, or polyvinyl pyrrolidone
• CPC Class Codes: A61K 47/36 Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
• CPC Class Codes: A61K 47/42 Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein oligopeptides having up to five amino acids A61K47/183; polyamino acids A61K47/34
• CPC Class Codes: A61K 9/5138 obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, poly(meth)acrylates
• CPC Class Codes: A61K 9/5192 Processes
• CPC Class Codes: B01D 15/3852 using imprinted phases or molecular recognition; using imprinted phases
• CPC Class Codes: B01J 20/261 obtained by reactions only involving carbon to carbon unsaturated bonds macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds per se C08F
• CPC Class Codes: B01J 20/267 Cross-linked polymers
• CPC Class Codes: B01J 20/268 Polymers created by use of a template, e.g. molecularly imprinted polymers
• CPC Class Codes: B01J 20/28007 with size in the range 1-100 nanometers, e.g. nanosized particles, nanofibers, nanotubes, nanowires or the like carbon nanostructures B01J20/205
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C07K 1/22 Affinity chromatography or related techniques based upon selective absorption processes
• CPC Class Codes: C08F 2/10 Aqueous solvent
• CPC Class Codes: C08F 2/44 Polymerisation in the presence of compounding ingredients, e.g. plasticisers, dyestuffs, fillers
• CPC Class Codes: G01N 2600/00 Assays involving molecular imprinted polymers/polymers created around a molecular template
• CPC Class Codes: G01N 33/5432 Liposomes or microcapsules
• CPC Class Codes: G01N 33/54346 Nanoparticles

Abstract

The present invention relates to imprinted polymer nanoparticles. In particular, the present invention provides imprinted polymer nanoparticles polymerized in the presence of a target molecule (e.g., peptide), wherein the imprinted polymer nanoparticles comprise vinyl, acryl, and/or methacryl monomers, wherein the monomers have affinity for the target molecule. The present invention also relates to methods of using imprinted polymer nanoparticles in biomacromolecular purification methods (e.g., to purify monoclonal antibodies or hormones), in toxin elimination methods (e.g., hemoperfusion), in diagnostics, in research, as well as in therapeutic methods (e.g., therapeutic methods where antisera or monoclonal antibodies are normally employed).

Claims

1. A method of making imprinted polymer nanoparticles comprising:

a) combining at least one type of monomers, a cross-linking agent, and target molecules in an aqueous medium to generate a reaction mixture, wherein said at least one type of monomer comprises N-tert-butylacrylamide (TBAm) momomers; and

b) incubating said reaction mixture under polymerization conditions such that imprinted polymer nanoparticles are generated that are specific for said target molecules.

2. The method of claim 1, wherein said at least one type of monomer further comprises acrylic acid monomers.

3. The method of claim 1, wherein said reaction mixture further comprises N-isopropylacrylamide (NIPAm) monomers.

4. The method of claim 1, wherein said target molecules are proteins.

5. The method of claim 1, wherein said target molecules are toxins.

6. The method of claim 1, wherein said target molecules are polysaccharides.

7. The method of claim 1, wherein said reaction mixture further comprises an ionic surfactant.

8. The method of claim 7, wherein said ionic surfactant comprises sodium dodecyl sulfate.

9. The method of claim 1, wherein said crosslinking agent comprises N,N′

-methylenebis(acrylamide).

10. The method of claim 1, wherein said at least one type of monomer further comprises acrylamide monomers.

11. The method of claim 1, wherein said crosslinking agent comprises N,N′

-methylenebis(acrylamide) and wherein said reaction mixture further comprises N-isopropylacrylamide (NIPAm) monomers.

12. The method of claim 11, wherein said at least one type of monomer further comprises acrylic acid monomers.