Method for extracorporeal removal of a pathogenic microbe, an inflammatory cell or an inflammatory protein from blood
First Claim
1. A method for treating a subject in need thereof, by extracorporeal removal of Staphylococcus aureus, said method comprising:
- a) contacting said subject'"'"'s whole blood with heparin immobilized on a solid substrate, said heparin having a terminal residue, wherein heparin immobilization consists of a single covalent link of said terminal residue to said solid substrate by covalent end-point attachment, under conditions allowing binding of said Staphylococcus aureus in said subject'"'"'s whole blood sample to the heparin;
b) separating the whole blood from the solid substrate;
c) recovering said whole blood containing a reduced amount of said Staphylococcus aureus; and
d) reintroducing into said subject said whole blood containing a reduced amount of said Staphylococcus aureus.
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Accused Products
Abstract
The present invention relates to a method for extracorporeal removal of a pathogenic microbe, an inflammatory cell or an inflammatory protein from mammalian blood/use of a device comprising a carbohydrate immobilized on a solid substrate, said carbohydrate having a binding affinity for a pathogenic microbe, an inflammatory cell or an inflammatory protein, for extracorporeal removal of said pathogenic microbe, inflammatory cell or inflammatory protein from mammalian blood/use of a carbohydrate having a binding affinity for a pathogenic microbe, an inflammatory cell or an inflammatory protein, wherein said carbohydrate is immobilized on a solid substrate, in the preparation of a device for treatment of a condition caused or aggravated by said pathogenic microbe, inflammatory cell or inflammatory protein/and a method for treatment of a mammalian subject suffering from a condition caused or aggravated by a pathogenic microbe, an inflammatory cell or an inflammatory protein.
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Citations
11 Claims
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1. A method for treating a subject in need thereof, by extracorporeal removal of Staphylococcus aureus, said method comprising:
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a) contacting said subject'"'"'s whole blood with heparin immobilized on a solid substrate, said heparin having a terminal residue, wherein heparin immobilization consists of a single covalent link of said terminal residue to said solid substrate by covalent end-point attachment, under conditions allowing binding of said Staphylococcus aureus in said subject'"'"'s whole blood sample to the heparin; b) separating the whole blood from the solid substrate; c) recovering said whole blood containing a reduced amount of said Staphylococcus aureus; and d) reintroducing into said subject said whole blood containing a reduced amount of said Staphylococcus aureus. - View Dependent Claims (2, 3, 4, 10)
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5. A method for extracorporeal removal of a pathogenic microbe, wherein said pathogenic microbe is Staphylococcus aureus, from human whole blood, said method comprising:
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a) contacting human whole blood with heparin immobilized on a solid substrate by blood flow through a device comprising said solid substrate, said heparin having a terminal residue, wherein heparin immobilization consists of a single covalent link of said terminal residue to said solid substrate by covalent end-point attachment, under conditions allowing binding of said pathogenic microbe, wherein said pathogenic microbe is Staphylococcus aureus in said human whole blood sample to the heparin; b) separating the whole blood from the solid substrate; and c) recovering said whole blood containing a reduced amount of said pathogenic microbe, wherein said pathogenic microbe is Staphylococcus aureus. - View Dependent Claims (6, 7, 8, 9, 11)
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Specification