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Differentiation of pluripotent stem cells

  • US 9,181,528 B2
  • Filed: 08/17/2011
  • Issued: 11/10/2015
  • Est. Priority Date: 08/31/2010
  • Status: Active Grant
First Claim
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1. A method to derive a population of pancreatic endocrine precursor cells from pluripotent stem cells comprising the steps of:

  • a. Culturing a population of pluripotent stem cells;

    b. Differentiating the population of pluripotent stem cells into a population of cells expressing markers characteristic of the definitive endoderm lineage;

    c. Differentiating the population of cells expressing markers characteristic of the definitive endoderm lineage into a population of primitive gut tube cells;

    d. Differentiating the population of primitive gut tube cells into a population of posterior foregut cells; and

    e. Treating the population of posterior foregut cells with a medium supplemented with a CYP26A inhibitor and with no added retinoic acid such that the population of posterior foregut cells differentiates into a population of pancreatic endocrine precursor cells, wherein expression of endocrine precursor markers is increased and wherein the differentiating in steps b., c. and d. comprises treatment in a medium lacking a CPY26A inhibitor.

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