Photocatalytic hydrogen production and polypeptides capable of same

• US 9,181,555 B2
• Filed: 07/23/2008
• Issued: 11/10/2015
• Est. Priority Date: 07/23/2007
• Status: Expired due to Fees

• Current Assignee: Ramot at Tel Aviv University Limited (Tel Aviv University)
• Original Assignee: Ramot at Tel Aviv University Limited (Tel Aviv University)
• Inventors: Yacoby, Iftach, Gazit, Ehud, Nelson, Nathan, Benhar, Itai
• Primary Examiner(s): Kallis, Russell
• Application Number:
  US12/670,407
• Publication Number:
  US 20100203609A1
• Time in Patent Office: 2,666 Days
• Field of Search: 435/257.1, 800/296
• US Class Current: 1/1
• CPC Class Codes: C12M 21/02 Photobioreactors culturing algae A01G33/00, A01H4/001, C12N1/12
• CPC Class Codes: C12M 21/18 Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
• CPC Class Codes: C12M 31/10 by light emitting elements located inside the reactor, e.g. LED or OLED
• CPC Class Codes: C12M 47/10 Separation or concentration of fermentation products bioreactors combined with means for distillation or extraction of liquid fuel C12M43/02
• CPC Class Codes: C12N 15/62 DNA sequences coding for fusion proteins
• CPC Class Codes: C12N 9/0067 acting on hydrogen as donor (1.12)
• CPC Class Codes: C12N 9/96 Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
• CPC Class Codes: C12P 3/00 Preparation of elements or inorganic compounds except carbon dioxide recovery of carbon dioxides as by-products C12F3/02
• CPC Class Codes: C12Y 112/98002 5,10-Methenyltetrahydromethanopterin hydrogenase (1.12.98.2)

Abstract

An isolated polypeptide comprising a hydrogen generating enzyme attached to a heterologous ferredoxin is disclosed, as well as polynucleotides encoding same, nucleic acid constructs capable of expressing same and cells expressing same. A method for generating hydrogen using the isolated polypeptide is also disclosed.

Claims

1. An isolated polypeptide comprising an algal Fe-only hydrogenase attached to an algal ferredoxin, comprising an amino acid sequence as set forth in SEQ ID NOs:

23, 25 or 27.

2. An isolated polynucleotide encoding the polypeptide of claim 1.

3. A cell comprising the isolated polynucleotide of claim 2.

4. The cell of claim 3, being selected from the group consisting of a cyanobacterial cell, an algal cell and a higher plant cell.

5. A method of generating hydrogen, the method comprising combining the polypeptide of claim 1 with an electron donor so as to generate an electron transfer chain, wherein said electron transfer chain is configured such that said electron donor is capable of donating electrons to the polypeptide of claim 1, thereby generating hydrogen.

6. The method of claim 5, wherein the generating hydrogen is effected under anaerobic conditions.

7. The method of claim 5, wherein said electron donor is selected from the group consisting of a biomolecule, a chemical, water, an electrode and a combination of the above.

8. The method of claim 7, wherein said biomolecule comprises Photosystem I (PSI) or rhodopsin.

9. The method of claim 5, further comprising harvesting the hydrogen following the generating.

10. The method of claim 5, wherein said combining is effected in a cell-free system.

11. The method of claim 5, wherein said combining is effected in a cellular system.

12. The method of claim 11, wherein said cellular system is selected from the group consisting of a cyanobacteria, an algae and a higher plant.

13. The method of claim 11, further comprising down-regulating an expression of endogenous ferredoxin in said cellular system.

14. The isolated polynucleotide of claim 2, comprising a nucleic acid sequence as set forth in SEQ ID NOs:

1, 3 or 5.