Systems and methods for the automated pre-treatment and processing of biological samples

• US 9,182,324 B2
• Filed: 01/06/2012
• Issued: 11/10/2015
• Est. Priority Date: 12/20/2002
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Dako Denmark A/S (Agilent Technologies, Inc.)
• Inventors: Feingold, Gordon Alan, Gilmartin, James B., Holbrook, Mark Richard, Favuzzi, John A., Key, Marc E.
• Primary Examiner(s): White, Dennis M
• Assistant Examiner(s): KILPATRICK, BRYAN T
• Application Number:
  US13/345,605
• Publication Number:
  US 20120179293A1
• Time in Patent Office: 1,404 Days
• Field of Search: 422/63, 700/245, 700/258, 901/46, 901/50
• US Class Current: 1/1
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 35/0092 Scheduling
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: G16H 10/40 for data related to laboratory analysis, e.g. patient specimen analysis
• CPC Class Codes: G16H 40/63 for local operation
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/110833 Utilizing a moving indicator strip or tape
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/112499 with sample on test slide
• CPC Class Codes: Y10T 436/114998 with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

Systems and methods in accordance with embodiments of the present invention allow for the automatic control and scheduling of a staining apparatus for biological samples on slides present within the apparatus. In some embodiments, the actions of a robot coupled to the staining apparatus, which performs some of the staining tasks on the individual slides in accordance with their respective protocols, may be prioritized and scheduled. In some embodiments, the scheduling may result in increasing or maximizing the throughput of slides. In some embodiments, robot scheduling ensures that the individual slides are processed substantially within the tolerances specified by their respective protocols. In some embodiments, the robot scheduler may respond to spontaneous user actions and adaptively schedule or re-schedule robot actions.

Claims

1. A method for scheduling robot tasks for at least one robot in an automated stainer system, wherein the at least one robot treats slides on the automated stainer system according to a treatment protocol with reagents on the automated stainer system, the method comprising:

establishing a connection over a network between a computer and the automated stainer system wherein the network is configured to connect to a plurality of individual automated stainer systems, wherein each of the plurality of individual automated stainer systems includes a network interface, a stain dispenser, and a robot;

obtaining from the automated stainer system an identity of a first slide in the automated stainer system via the network;

selecting a first treatment protocol based on the identity of the first slide;

sending, over the network, first treatment protocol to be performed on the first slide by the automated stainer system;

creating a first robot task list from the first treatment protocol comprising robot tasks to be performed on the first slide by the at least one robot, wherein the robot is configured to dispense, without interruption, onto the first slide at least one reagent from a reagent bottle while another slide, different from the first slide, is added or removed from the automated stainer system during the first treatment protocol;

monitoring, over the network, processing performed on the first slide according to the first robot task list;

obtaining from the automated stainer system an identity of a second slide inserted into the automated stainer system during processing of the first slide;

sending, over the network, a second treatment protocol to be performed on the second slide by the automated stainer system.

2. The method of claim 1, wherein the network is a wide area network.

3. The method of claim 1, wherein the first treatment protocol includes a target retrieval protocol.

4. The method of claim 2, further comprising:

sending, over the network, a first altered treatment protocol to be performed on the first slide by the automated stainer system;

treating, by the robot, the first slide according to the first altered treatment protocol.

5. The method of claim 4, further comprising:

designating a processing priority of at least one of the first slide and the second slide; and

determining the first altered treatment protocol according to the designated processing priority of at least one of the first slide and the second slide.

6. The method of claim 2, further comprising:

simulating operation of the automated stainer system; and

determining the second treatment protocol according to results of the simulation.

7. The method of claim 2, further comprising calculating a completion time of at least one of the first slide and the second slide.

8. The method of claim 7, wherein the processing priority of at least one of the first slide and the second slide is determined according to a user request.

9. The method of claim 2, further comprising establishing a connection over the network between the computer and a plurality of automated stainer systems.

10. The method of claim 9, further comprising coordinating robot task lists of the plurality of automated stainer systems.

11. The method of claim 2, further comprising obtaining the first treatment protocol from a database available to the network.

12. The method of claim 2, further comprising monitoring consumable usage by the automated stainer system.

13. The method of claim 2, further comprising receiving from the automated stainer system information including information about slides, process-status, treatment protocols, reagents, fluids, and diagnostics.