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Microscale western blot

  • US 9,182,371 B2
  • Filed: 08/23/2012
  • Issued: 11/10/2015
  • Est. Priority Date: 02/26/2010
  • Status: Active Grant
First Claim
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1. A capillary electrophoresis and blotting system comprising:

  • an electrophoresis component comprising a separation capillary having proximal and distal ends, the separation capillary operable to be filled with a sieving medium and electrophorese a sample from the proximal end to the distal end;

    a blotting component comprising a surface;

    a translating component operable to change position of the distal end of the separation capillary relative to the surface of the blotting component, wherein the distal end of the separation capillary and the surface of the blotting component are operable to be electrically coupled during electrophoresis of the sample; and

    a buffer capillary having proximal and distal ends, the distal end of the buffer capillary located proximate to the distal end of the separation capillary, wherein the buffer capillary is operable to be filled with electrophoresis buffer or gel to electrically couple the distal end of the separation capillary and the surface of the blotting component during electrophoresis of the sample.

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