Microscale western blot
First Claim
Patent Images
1. A capillary electrophoresis and blotting system comprising:
- an electrophoresis component comprising a separation capillary having proximal and distal ends, the separation capillary operable to be filled with a sieving medium and electrophorese a sample from the proximal end to the distal end;
a blotting component comprising a surface;
a translating component operable to change position of the distal end of the separation capillary relative to the surface of the blotting component, wherein the distal end of the separation capillary and the surface of the blotting component are operable to be electrically coupled during electrophoresis of the sample; and
a buffer capillary having proximal and distal ends, the distal end of the buffer capillary located proximate to the distal end of the separation capillary, wherein the buffer capillary is operable to be filled with electrophoresis buffer or gel to electrically couple the distal end of the separation capillary and the surface of the blotting component during electrophoresis of the sample.
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Abstract
Systems and methods are provided for integrating the electrophoresis and blotting of samples. A capillary electrophoresis and blotting system allows concomitant electrophoretic separation and blotting to provide a rapid and simplified process. A microfluidic electrophoresis and blotting system provides electrophoretic separation in a microfluidic channel followed by electroblotting from the microfluidic channel to also provide a rapid and simplified process. These systems and methods can be used to assay smaller amounts of sample in less time than conventional processes, including conventional Western blotting techniques.
9 Citations
38 Claims
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1. A capillary electrophoresis and blotting system comprising:
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an electrophoresis component comprising a separation capillary having proximal and distal ends, the separation capillary operable to be filled with a sieving medium and electrophorese a sample from the proximal end to the distal end; a blotting component comprising a surface; a translating component operable to change position of the distal end of the separation capillary relative to the surface of the blotting component, wherein the distal end of the separation capillary and the surface of the blotting component are operable to be electrically coupled during electrophoresis of the sample; and a buffer capillary having proximal and distal ends, the distal end of the buffer capillary located proximate to the distal end of the separation capillary, wherein the buffer capillary is operable to be filled with electrophoresis buffer or gel to electrically couple the distal end of the separation capillary and the surface of the blotting component during electrophoresis of the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A microfluidic electrophoresis and blotting system comprising:
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an electrophoresis component comprising a microfluidic channel and a post-column channel, the microfluidic channel having proximal and distal ends and operable to be filled with a sieving medium and electrophorese a sample from the proximal end toward the distal end, the post-column channel having proximal and distal ends and operable to be filled with an electrophoresis buffer or gel, wherein the distal end of the microfluidic channel is coupled to the proximal end of the post-column channel; a blotting component comprising a surface, wherein the surface of the blotting component comprises a blotting membrane; and a translating component operable to change position of the distal end of the post-column channel relative to the surface of the blotting component, wherein the distal end of the post-column channel and the surface of the blotting component are operable to be electrically coupled during electrophoresis of the sample. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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Specification