Microfluidic test carrier for apportioning a liquid quantity into subquantities

• US 9,186,671 B2
• Filed: 04/26/2013
• Issued: 11/17/2015
• Est. Priority Date: 10/28/2010
• Status: Active Grant

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Augstein, Manfred, Boehm, Christoph, Wuerl, Susanne
• Primary Examiner(s): Alexander, Lyle
• Assistant Examiner(s): Gerido, Dwan A
• Application Number:
  US13/871,720
• Publication Number:
  US 20130236376A1
• Time in Patent Office: 935 Days
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 2300/0806 Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 2300/0851 Bottom walls
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001

Abstract

A microfluidic test carrier having a substrate, covering layer, and capillary structure formed in the substrate is provided. The capillary structure is enclosed by the substrate and covering layer and comprises a receiving chamber, sample chamber and connection channel between the receiving and sample chambers. The receiving chamber has two boundary surfaces and a side wall, wherein one boundary surface forms the bottom and the other forms the cover. The receiving chamber has a surrounding venting channel and dam between the receiving chamber and venting channel. The dam and venting channel form a capillary stop configured as a geometric valve, through which air from the receiving chamber can escape into the venting channel. The connecting channel between the venting channel outflow and sample chamber inflow controls fluid transport from the receiving chamber into the sample chamber. The capillary stop is configured to prevent autonomous fluid transport from the receiving chamber.

Claims

1. A microfluidic test carrier for apportioning a quantity of fluid into sub-quantities, said test carrier comprising:

a substrate and a covering layer, and having a capillary structure formed in the substrate that is enclosed by the substrate and the covering layer, wherein

the capillary structure comprises a receiving chamber, a sample chamber and a connecting channel between the receiving chamber and the sample chamber;

the receiving chamber has two opposite boundary surfaces and a side wall, wherein one of the boundary surfaces has an inlet port and one of the boundary surfaces is a the floor of the receiving chamber and the other boundary surface is a the cover of the receiving chamber;

the receiving chamber has an inner zone, a circumferential venting channel, and a circumferential dam positioned horizontally between the inner zone receiving chamber and the venting channel, in which wherein the dam is closer to the inlet port than to the venting channel to the inlet port;

the dam is configured such that a capillary stop forming a geometric valve is formed by the dam and the venting channel, and in which the geometric valve is a capillary stop for fluid, but through which air can escape out of the venting channel; and

the connecting channel extends between an outflow orifice of the venting channel and an inlet orifice of the sample chamber such that fluid transport is possible from the receiving chamber into the sample chamber, and

in which the valve prevents is configured to prevent automatic fluid transport out of the receiving chamber.

2. The microfluidic test carrier according to claim 1, wherein a center point of the receiving chamber is positioned eccentrically with respect to the center point of the test carrier or to the center of gravity of the test carrier.

3. The microfluidic test carrier according to claim 1, wherein the floor of the receiving chamber is dished such that a depression is formed in a collecting region close to the outflow orifice, into which the fluid flows.

4. The microfluidic test carrier according to claim 1, wherein the side wall is provided with an indentation, which extends away from the center point of the receiving chamber, and the outflow orifice is arranged in the indentation.

5. The microfluidic test carrier according to claim 1, wherein a plurality of outflow orifices are positioned in the venting channel, which are each in fluid communication with a connecting channel that extends away from the receiving chamber.

6. The microfluidic test carrier according to claim 5, wherein the outflow orifices are equidistantly distributed about the circumference of the venting channel.

7. The microfluidic test carrier according to claim 1, wherein the sample chamber is provided with an outlet orifice that is in fluid communication with an outlet channel such that a fluid can flow out of the sample chamber.

8. The microfluidic test carrier according to claim 7, wherein a further fluid chamber with an inlet orifice connects with the outlet channel, which is in fluid communication with the sample chamber by means of the outlet channel.

9. The microfluidic test carrier according to claim 1, wherein one of the boundary surfaces of the receiving chamber is curved.

10. The microfluidic test carrier according to claim 9, wherein the curved boundary surface is the cover of the receiving chamber.

11. The microfluidic test carrier according to claim 9, wherein the curved boundary surface is the floor of the receiving chamber.

12. The microfluidic test carrier according to claim 11, wherein the floor is curved such that the floor is upwardly inclined towards the dam of the receiving chamber.

13. The microfluidic test carrier according to claim 1, wherein the test carrier rotates about a rotational axis that extends through the test carrier.

14. The microfluidic test carrier according to claim 13, wherein the rotational axis extends through the center point or the center of gravity of the test carrier.

15. The microfluidic test carrier according to claim 13, wherein the receiving chamber is positioned in the test carrier such that the rotational axis extends through the inlet port.

16. The microfluidic test carrier according to claim 13, wherein the receiving chamber is positioned in the test carrier such that the rotational axis extends through the center point or the center of gravity of the receiving chamber.

17. The microfluidic test carrier according to claim 13, wherein a boss is formed on the floor of the receiving chamber which is positioned facing the inlet port in the cover.

18. The microfluidic test carrier according to claim 17, wherein the boss is conical.

19. The microfluidic test carrier according to claim 17, wherein the boss is aligned with the rotational axis.