Chromobacterium bioactive compositions and metabolites

• US 9,187,531 B2
• Filed: 03/14/2014
• Issued: 11/17/2015
• Est. Priority Date: 10/25/2010
• Status: Active Grant

• Current Assignee: Marrone Bio Innovations Inc. (Bioceres Crop Solutions Corp.)
• Original Assignee: Marrone Bio Innovations Inc. (Bioceres Crop Solutions Corp.)
• Inventors: Asolkar, Ratnakar, Huang, Huazhang, Koivunen, Marja, Marrone, Pamela
• Primary Examiner(s): Tate, Chris R
• Assistant Examiner(s): FIEBIG, RUSSELL G
• Application Number:
  US14/213,896
• Publication Number:
  US 20140199269A1
• Time in Patent Office: 613 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A01N 2300/00 Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
• CPC Class Codes: A01N 43/22 rings with more than six members
• CPC Class Codes: A01N 43/56 1,2-Diazoles; Hydrogenated 1,2-diazoles
• CPC Class Codes: A01N 43/90 having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
• CPC Class Codes: A01N 47/06 containing —O—CO—O— groups; Thio analogues thereof
• CPC Class Codes: A01N 53/00 Biocides, pest repellants or attractants, or plant growth regulators containing cyclopropane carboxylic acids or derivatives thereof
• CPC Class Codes: A01N 63/20 Bacteria; Substances produced thereby or obtained therefrom
• CPC Class Codes: A61K 2236/11 involving culturing conditions, e.g. cultivation in the dark or under defined water stress
• CPC Class Codes: A61K 2236/30 Extraction of the material
• CPC Class Codes: A61K 35/66 Microorganisms or materials therefrom fungi, yeasts or candida A61K36/06
• CPC Class Codes: A61K 35/74 Bacteria therapeutic use of a bacterial protein A61K38/00
• CPC Class Codes: C07K 5/0205 containing the structure -NH-(X)3-C(=0)-, e.g. statine or derivatives thereof
• CPC Class Codes: C07K 7/64 Cyclic peptides containing only normal peptide links
• CPC Class Codes: C12P 17/188 Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms ergot-alcaloids C12P17/183
• CPC Class Codes: C12P 21/02 having a known sequence of two or more amino acids, e.g. glutathione
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Provided are bioactive compounds and metabolites derived from Chromobacterium species culture responsible for controlling pests, compositions containing these compounds, methods for obtaining these compounds and methods of using these compounds and compositions for controlling pests.

Claims

1. A method for reducing root-knot nematode infestation in a plant in need thereof comprising

applying to the plant, one or more seeds of the plant, and/or a substrate in which the plant is grown, an amount of a whole cell broth of an isolated strain of a Chromobacterium subtsugae sp. nov. (NRRL B-30655), or a supernatant, filtrate or extract of the whole cell broth, effective to reduce said root-knot nematode infestation.

2. The method of claim 1, wherein the method comprises applying the whole cell broth of an isolated strain of a Chromobacterium subtsugae sp. nov. (NRRL B-30655).

3. The method of claim 1, wherein the method comprises applying the supernatant, filtrate, or extract of the whole cell broth.

4. The method of claim 1, wherein the whole cell broth or the supernatant, filtrate, or extract of the whole cell broth is applied to the substrate and the substrate is soil.

5. The method of claim 4, wherein the whole cell broth or the supernatant, filtrate, and/or extract of the whole cell broth is applied by soil drench.

6. The method of claim 1, wherein the whole cell broth or the supernatant, filtrate, or extract of the whole cell broth is applied to the plant.

7. The method of claim 1, wherein the whole cell broth or the supernatant, filtrate, or extract of the whole cell broth is applied to the one or more seeds.

8. A method for reducing root-knot nematode infestation in a plant in need thereof comprising applying to the plant, one or more seeds of the plant, and/or a substrate in which the plant is grown, composition consisting essentially of a whole cell broth of an isolated strain of a Chromobacterium subtsugae sp. nov. (NRRL B-30655), in an amount effective to reduce said root-knot nematode infestation.

9. The method of claim 8, wherein the composition is applied to the plant.

10. The method of claim 8, wherein the composition is applied to the one or more seeds.

11. The method of claim 8, wherein the composition is applied to the substrate and the substrate is soil.