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Glycosylation of peptides via O-linked glycosylation sequences

  • US 9,187,532 B2
  • Filed: 07/23/2007
  • Issued: 11/17/2015
  • Est. Priority Date: 07/21/2006
  • Status: Active Grant
First Claim
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1. A method comprising expressing a sequon polypeptide in a host cell, wherein said sequon polypeptide corresponds to a parent polypeptide and comprises an exogenous O-linked glycosylation sequence comprising the amino acid sequence of SEQ ID NO:

  • 1;


    (X)mPO*U(B)p(Z)r(J)s(O)t(P)n



    (SEQ ID NO;

         1)whereinm, n, and p are integers independently selected from the group consisting of 0 and 1;

    r, s, and t are 0;

    P is proline;

    O* is serine (S) or threonine (T);

    U is a member selected from the group consisting of proline (P), glutamic acid (E), glutamine (Q), asparagine (N), threonine (T), glycine (G), and alanine (A);

    X and B are members independently selected from the group consisting of glutamic acid (E), glutamine (Q), aspartic acid (D), asparagine (N), threonine (T), and serine (S); and

    Z, J and O are members independently selected from the group consisting of glutamic acid (E), glutamine (Q), aspartic acid (D), asparagine (N), threonine (T), serine (S), tyrosine (Y), and methionine (M),wherein the O-linked glycosylation is at O*, andwherein the parent polypeptide is selected from the group consisting of bone morphogenetic protein 2 (BMP-2), bone morphogenetic protein 7 (BMP-7), bone morphogenetic protein 15 (BMP-15), neurotrophin-3 (NT-3), von Willebrand factor (vWF) protease, α

    1-antitrypsin (α

    -1 protease inhibitor), tissue-type plasminogen activator (TPA), human chorionic gonadotropin (hCG), anti-thrombin III (AT III), follicle stimulating hormone (FSH), glucagon-like peptide-2 (GLP-2), Factor VII, Factor VIII, B-domain deleted Factor VIII, Factor IX, Factor X, and Factor XIII.

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