Single particle detection device, single particle detection method, and computer program for single particle detection, using optical analysis

• US 9,188,535 B2
• Filed: 09/25/2014
• Issued: 11/17/2015
• Est. Priority Date: 04/18/2012
• Status: Active Grant

• Current Assignee: Olympus Corporation
• Original Assignee: Olympus Corporation
• Inventors: Hanashi, Takuya
• Primary Examiner(s): Gaworecki, Mark R
• Application Number:
  US14/496,177
• Publication Number:
  US 20150108369A1
• Time in Patent Office: 418 Days
• Field of Search: 250/458.1, 250/459.1
• US Class Current: 1/1
• CPC Class Codes: G01N 15/1429 Signal processing
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 2015/1486 Counting the particles
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16
• CPC Class Codes: G01N 2201/06113 Coherent sources; lasers
• CPC Class Codes: G02B 21/0064 multi-spectral or wavelength-selective arrangements, e.g. wavelength fan-out, chromatic profiling G02B21/0076 takes precedence
• CPC Class Codes: G02B 21/0076 arrangements using fluorescence or luminescence
• CPC Class Codes: G02B 21/0084 time-scale detection, e.g. strobed, ultra-fast, heterodyne detection

Abstract

There is provided a single particle detection technique based on the scanning molecule counting method which individually detects single particles using light measurement with a confocal or multiphoton microscope, where the existences of a non-light-emitting particle and a light-emitting particle can be detected while being discriminated from one another in a sample solution. The inventive technique of detecting a single particle detects light from a light detection region during moving the position of the light detection region of the microscope in a sample solution containing a non-light-emitting particle and a light-emitting particle to generate time series light intensity data; and detects in the time series light intensity data a light intensity increase relative to background light intensity as a signal indicating the existence of the light-emitting particle and a light intensity reduction relative to the background light intensity as a signal indicating the existence of the non-light-emitting particle.

Claims

1. A single particle detection device which detects single particles dispersed and moving at random in a sample solution using an optical system of a confocal microscope or a multiphoton microscope, comprising:

a light detection region mover which moves a position of a light detection region of the optical system in the sample solution;

a light detector which detects light from the light detection region; and

a signal processor which generates time series light intensity data of the light from the light detection region detected with the light detector during moving the position of the light detection region in the sample solution and detects a signal indicating an existence of each single particle individually in the time series light intensity data;

wherein the light from the light detection region detected by the light detector includes substantially constant background light;

the single particles include a first single particle having an emitting light intensity higher than the background light and a second single particle having an emitting light intensity lower than the background light;

the signal of the first single particle is an increase of the light intensity detected by the light detector occurring when the first single particle enters into the light detection region; and

the signal of the second single particle is a reduction of the light intensity detected by the light detector occurring when the second single particle enters into the light detection region.

2. The device of claim 1, wherein the signal processor counts numbers of the individually detected signals indicating existences of the first and second particles separately to count numbers of the first and second single particles detected during the moving of the position of the light detection region.

3. The device of claim 1, wherein the emitted light intensity emitted from the first single particle per unit volume is higher than the background light intensity emitted from the inside of the light detection region per unit volume;

and the emitted light intensity emitted from the second single particle per unit volume is lower than the background light intensity emitted from the inside of the light detection region per unit volume.

4. The device of claim 1, wherein the background light is fluorescence, phosphorescence, chemiluminescence, bioluminescence or scattered light owing to substance dispersed in the sample solution, or illumination light.

5. A single particle detection method of detecting single particles dispersed and moving at random in a sample solution using an optical system of a confocal microscope or a multiphoton microscope, comprising steps of:

moving a position of a light detection region of the optical system in the sample solution;

detecting light from the light detection region during moving the position of the light detection region in the sample solution, and generating time series light intensity data; and

detecting in the time series light intensity data individually a signal indicating an existence of each single particle;

wherein the detected light from the light detection region includes substantially constant background light;

the single particles include a first single particle having an emitting light intensity higher than the background light and a second single particle having an emitting light intensity lower than the background light;

the signal of the first single particle is an increase of the light intensity detected by the light detector occurring when the first single particle enters into the light detection region; and

the signal of the second single particle is a reduction of the light intensity detected by the light detector occurring when the second single particle enters into the light detection region.

6. The method of claim 5, further comprising a step of counting numbers of the individually detected signals indicating existences of the first and second particles separately to count the numbers of the first and second single particles detected during the moving of the position of the light detection region.

7. The method of claim 5, wherein the emitted light intensity emitted from the first single particle per unit volume is higher than the background light intensity emitted from the inside of the light detection region per unit volume;

and the emitted light intensity emitted from the second single particle per unit volume is lower than the background light intensity emitted from the inside of the light detection region per unit volume.

8. The method of claim 5, wherein the background light is fluorescence, phosphorescence, chemiluminescence, bioluminescence or scattered light owing to substance dispersed in the sample solution, or illumination light.

9. A non-transitory computer readable storage device having a computer program product including programmed instructions for optical analysis of detecting light from single particles dispersed and moving at random in a sample solution using an optical system of a confocal microscope or a multiphoton microscope, said programmed instructions causing a computer to perform steps comprising:

moving a position of a light detection region of the optical system in the sample solution;

detecting light from the light detection region during moving the position of the light detection region in the sample solution, and generating time series light intensity data; and

detecting in the time series light intensity data individually a signal indicating an existence of each single particle;

wherein the detected light from the light detection region includes substantially constant background light;

the single particles include a first single particle having an emitting light intensity higher than the background light and a second single particle having an emitting light intensity lower than the background light;

the signal of the first single particle is an increase of the light intensity detected by the light detector occurring when the first single particle enters into the light detection region; and

the signal of the second single particle is a reduction of the light intensity detected by the light detector occurring when the second single particle enters into the light detection region.

10. The non-transitory computer readable storage device of claim 9, further comprising a step of counting numbers of the individually detected signals indicating existences of the first and second particles separately to count the numbers of the first and second single particles detected during the moving of the position of the light detection region.

11. The non-transitory computer readable storage device of claim 10, wherein the emitted light intensity emitted from the first single particle per unit volume is higher than the background light intensity emitted from the inside of the light detection region per unit volume;

and the emitted light intensity emitted from the second single particle per unit volume is lower than the background light intensity emitted from the inside of the light detection region per unit volume.

12. The non-transitory computer readable storage device of claim 9, wherein the background light is fluorescence, phosphorescence, chemiluminescence, bioluminescence or scattered light owing to substance dispersed in the sample solution, or illumination light.