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Sensing method and sensing device

  • US 9,188,537 B2
  • Filed: 03/05/2012
  • Issued: 11/17/2015
  • Est. Priority Date: 03/29/2011
  • Status: Expired due to Fees
First Claim
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1. A sensing method of quantifying a concentration of analytes based on an intensity of fluorescence, which changes due to an interaction between the analytes and a labeled compound, comprising:

  • an acquisition step of acquiring the intensity of fluorescence at a predetermined quantification time using a fluorescence sensor;

    a selection step of selecting a concentration quantification rule from a steady concentration quantification rule in relation to the intensity of fluorescence and a non-steady concentration quantification rule representative of a relationship between the acquired intensity of fluorescence and a time derivative of the intensity of fluorescence at the predetermined quantification time, depending on a time-dependent change in the acquired intensity of fluorescence and/or an ambient temperature; and

    a quantification step of quantifying the concentration of the analytes according to the selected concentration quantification rule.

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