Sensing method and sensing device

• US 9,188,537 B2
• Filed: 03/05/2012
• Issued: 11/17/2015
• Est. Priority Date: 03/29/2011
• Status: Expired due to Fees

• Current Assignee: Terumo Kabushiki Kaisha (Terumo Corporation)
• Original Assignee: Terumo Kabushiki Kaisha (Terumo Corporation)
• Inventors: Fukai, Toshio, Matsumoto, Atsushi, Matsumoto, Kazuya, Suyama, Takuro
• Primary Examiner(s): Wallenhorst, Maureen
• Application Number:
  US14/007,403
• Publication Number:
  US 20140017799A1
• Time in Patent Office: 1,352 Days
• Field of Search: 436/63, 436/95, 436/164, 436/172, 436/147, 422/82.05, 422/82.08, 422/82.12, 435/14, 600/316, 600/317, 600/365
• US Class Current: 1/1
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/643 non-biological material
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

This invention relates to a sensing method and a sensing device for quantifying the concentration of an analyte by using the property that interaction between an analyte and a labeled compound changes fluorescence intensity. A fluorescence sensor is used to acquire fluorescence intensity at predetermined quantification time points. Then, the concentration of an analyte is quantified in accordance with a non-steady concentration quantification law including the relationship between the acquired fluorescence intensity and the time derivative quantity thereof.

Claims

1. A sensing method of quantifying a concentration of analytes based on an intensity of fluorescence, which changes due to an interaction between the analytes and a labeled compound, comprising:

an acquisition step of acquiring the intensity of fluorescence at a predetermined quantification time using a fluorescence sensor;

a selection step of selecting a concentration quantification rule from a steady concentration quantification rule in relation to the intensity of fluorescence and a non-steady concentration quantification rule representative of a relationship between the acquired intensity of fluorescence and a time derivative of the intensity of fluorescence at the predetermined quantification time, depending on a time-dependent change in the acquired intensity of fluorescence and/or an ambient temperature; and

a quantification step of quantifying the concentration of the analytes according to the selected concentration quantification rule.

2. The sensing method according to claim 1, wherein the non-steady concentration quantification rule is determined based on a chemical reaction formula representative of a bond dissociation reaction between the analytes and the labeled compound.

3. The sensing method according to claim 1, wherein the selection step selects the steady concentration quantification rule if the ambient temperature exceeds a first threshold value, and selects the non-steady concentration quantification rule if the ambient temperature does not exceed the first threshold value.

4. The sensing method according to claim 1, wherein the selection step selects the steady concentration quantification rule if the time-dependent change in the intensity of fluorescence does not exceed a second threshold value, and selects the non-steady concentration quantification rule if the time-dependent change in the intensity of fluorescence exceeds the second threshold value.

5. The sensing method according to claim 1, wherein the selection step further selects the concentration quantification rule from a plurality of non-steady concentration quantification rules having different degrees of contribution of the time derivative.

6. The sensing method according to claim 5, wherein the selection step increases the degrees of contribution as the ambient temperature decreases.

7. The sensing method according to claim 5, wherein the selection step increases the degrees of contribution as the time-dependent change in the intensity of fluorescence increases.

8. The sensing method according to claim 1, further comprising:

a changing step of changing a sampling interval for the intensity of fluorescence depending on the time-dependent change in the intensity of fluorescence and/or the ambient temperature.

9. The sensing method according to claim 1, further comprising:

a correction step of correcting the quantified concentration of the analytes depending on a degree of permeation of the analytes into the fluorescence sensor.

10. A sensing device for quantifying a concentration of analytes based on an intensity of fluorescence, which changes due to an interaction between the analytes and a labeled compound, comprising:

a fluorescence sensor for acquiring the intensity of fluorescence at a predetermined quantification time;

a quantification rule selector for selecting a concentration quantification rule from a steady concentration quantification rule in relation to the intensity of fluorescence and a non-steady concentration quantification rule representative of a relationship between the intensity of fluorescence acquired by the fluorescence sensor and a time derivative of the intensity of fluorescence at the predetermined quantification time, depending on a time-dependent change in the intensity of fluorescence acquired by the fluorescence sensor and/or an ambient temperature; and

a concentration quantifier for quantifying the concentration of the analytes according to the concentration quantification rule selected by the quantification rule selector.

11. The sensing device according to claim 10, wherein the non-steady concentration quantification rule is determined based on a chemical reaction formula representative of a bond dissociation reaction between the analytes and the labeled compound.

12. The sensing device according to claim 10, wherein the quantification rule selector selects the steady concentration quantification rule if the ambient temperature exceeds a first threshold value, and selects the non-steady concentration quantification rule if the ambient temperature does not exceed the first threshold value.

13. The sensing device according to claim 10, wherein the quantification rule selector selects the steady concentration quantification rule if the time-dependent change in the intensity of fluorescence does not exceed a second threshold value, and selects the non-steady concentration quantification rule if the time-dependent change in the intensity of fluorescence exceeds the second threshold value.

14. The sensing device according to claim 10, wherein the quantification rule selector further selects the concentration quantification rule from a plurality of non-steady concentration quantification rules having different degrees of contribution of the time derivative.

15. The sensing device according to claim 14, wherein the quantification rule selector increases the degrees of contribution as the ambient temperature decreases.

16. The sensing device according to claim 14, wherein the quantification rule selector increases the degrees of contribution as the time-dependent change in the intensity of fluorescence increases.

17. The sensing device according to claim 10, further comprising;

a sampling interval changer for changing a sampling interval for the intensity of fluorescence depending on the time-dependent change in the intensity of fluorescence and/or the ambient temperature.

18. The sensing device according to claim 10, wherein the concentration quantifier corrects the quantified concentration of the analytes depending on a degree of permeation of the analytes into the fluorescence sensor.