Chromatography apparatus having diffusion bonded coupler

• US 9,188,571 B2
• Filed: 08/23/2012
• Issued: 11/17/2015
• Est. Priority Date: 08/26/2011
• Status: Active Grant

• Current Assignee: Waters Technologies Corporation (Waters Corporation)
• Original Assignee: Waters Technologies Corporation (Waters Corporation)
• Inventors: Michienzi, Joseph D.
• Primary Examiner(s): CLEMENTE, ROBERT ARTHUR
• Application Number:
  US14/233,033
• Publication Number:
  US 20140157988A1
• Time in Patent Office: 1,181 Days
• US Class Current: 1/1
• CPC Class Codes: B01D 15/10 characterised by constructional or operational features
• CPC Class Codes: B01D 15/1871 placed in series
• CPC Class Codes: B01D 15/22 relating to the construction of the column
• CPC Class Codes: B05B 5/16 Arrangements for supplying liquids or other fluent material
• CPC Class Codes: G01N 30/60 Construction of the column
• CPC Class Codes: G01N 30/6004 end pieces
• CPC Class Codes: G01N 30/6026 Fluid seals
• CPC Class Codes: G01N 30/603 retaining the stationary phase, e.g. Frits
• CPC Class Codes: G01N 30/6039 in series
• CPC Class Codes: G01N 30/6052 body
• CPC Class Codes: G01N 30/6095 Micromachined or nanomachined, e.g. micro- or nanosize
• CPC Class Codes: G01N 30/7266 by electric field, e.g. electrospray
• CPC Class Codes: Y10T 29/49908 Joining by deforming

Abstract

Described is a chromatography a chromatography apparatus that includes a microfluidic substrate, a coupler and a sealing fitting. The microfluidic substrate can include one or more chromatography columns, such as analytical columns or trap columns, and an inlet in communication with the one or more chromatography columns. The coupler is diffusion bonded to the surface of the microfluidic substrate so that an opening in the coupler is aligned to the inlet in the microfluidic substrate. The sealing fitting includes a heat-resistant sealant disposed in the opening of the coupler and in contact with the surface of the substrate. The coupler and sealing fitting enable a compact fluid-tight connection to the inlet of the microfluidic substrate that exhibits high tensile and torsional strength, and does not require the use of adhesives.

Claims

1. A chromatography apparatus, comprising:

a microfluidic substrate comprising a ceramic material having a chromatography column therein, the ceramic material having a flat surface with an inlet therein that is in fluidic communication with the chromatography column;

a coupler comprising a metal body with an opening therethrough, the metal body having a flat surface that is diffusion bonded to the flat surface of the microfluidic substrate so that the opening in the coupler is aligned to the inlet of the microfluidic substrate; and

a sealing fitting comprising a heat-resistant sealant disposed in the opening of the coupler and being in contact with the flat surface of the substrate.

2. The chromatography apparatus of claim 1 wherein the metal body comprises titanium.

3. The chromatography apparatus of claim 1 wherein the heat-resistant sealant is a heat-resistant polymer.

4. The chromatography apparatus of claim 1 wherein the microfluidic substrate has a heating element to heat the microfluidic substrate to at least 300°

C.

5. The chromatography apparatus of claim 1 wherein the heat-resistant polymer is graphite loaded poly-oxydiphenylene-pyromellitimide.

6. The chromatography apparatus of claim 1 wherein the sealing fitting comprises graphite.

7. The chromatography apparatus of claim 1 further comprising a fitting in communication with the sealing fitting and configured to dispose a conduit in fluidic communication with the inlet of the microfluidic substrate.

8. The chromatography apparatus of claim 7 wherein the fitting is configured to receive a sleeve around a portion of the conduit.

9. The chromatography apparatus of claim 7 wherein the fitting has a ferrule portion at one end through which the conduit extends to be in fluidic communication with the inlet of the microfluidic substrate.

10. The chromatography apparatus of claim 9 further comprising a connector having an opening therethrough to pass the ferrule portion of the fitting and to press the ferrule portion of the fitting against the sealing fitting.

11. The chromatography apparatus of claim 10 wherein the sealing fitting has a passageway therein to receive the ferrule portion of the fitting and wherein the conduit that extends from the ferrule portion has an end that substantially abuts the inlet of the microfluidic substrate to form a fluidic connection, wherein the sealing fitting is pressed by the fitting against the flat surface of the substrate to achieve a fluid-tight seal at the inlet of the substrate.

12. A chromatographic apparatus, comprising:

a microfluidic substrate comprising a ceramic material having a chromatography column therein, the ceramic material having a surface with an inlet therein that is in fluidic communication with the chromatography column;

a coupler comprising a metal body having a hollow cylindrical shape, the metal body having a flat surface that is diffusion bonded to the surface of the microfluidic substrate in a location so that the metal body is aligned to the inlet of the microfluidic substrate;

a sealing fitting comprising a heat-resistant polymer and disposed in the metal body of the coupler in contact with the flat surface of the substrate, the sealing fitting having a passageway to pass a conduit;

a connector having an opening therethrough and being secured to the coupler, the connector configured to urge the sealing fitting toward the flat surface of the ceramic material of the microfluidic structure; and

a fitting secured to the connector and having a ferrule portion at one end through which the conduit extends to pass through the passageway of the sealing fitting and to be in fluidic communication with the inlet of the microfluidic substrate.