Device and method for indirect modulation of detection environment

• US 9,188,585 B2
• Filed: 05/13/2010
• Issued: 11/17/2015
• Est. Priority Date: 05/13/2010
• Status: Active Grant

• Current Assignee: Robert Bosch GmbH
• Original Assignee: Robert Bosch GmbH
• Inventors: Kavusi, Sam, Chen, Yi Wei, Chen, Michael, Lang, Christoph
• Primary Examiner(s): Sasaki, Shogo
• Application Number:
  US12/779,687
• Publication Number:
  US 20110278258A1
• Time in Patent Office: 2,014 Days
• Field of Search: 436/180, 422/107, 422/108, 422501-504, 422/68.1, 422402-404, 435/287.1, 435/288.4, 435/288.5
• US Class Current: 1/1
• CPC Class Codes: G01N 33/54306 Solid-phase reaction mechanisms
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

A system and method of indirectly modifying an environmental condition at a test site in one embodiment includes providing a test site on a substrate, providing a first activatable stimulant at the test site, providing an actuator configured to activate the first activatable stimulant at the test site, controlling the actuator to activate the first activatable stimulant, and modifying the local chemical environment at the test site with the first activated stimulant.

Claims

1. A system for controlling a chemical environmental condition at an affinity based test site comprising:

a substrate including at least one test site;

at least one activatable stimulant on the substrate and configured for use at the at least one test site; and

at least one actuator positioned to actuate at least one of the at least one activatable stimulant at the at least one test site such that chemical conditions at the at least one test site are altered thereby modifying an affinity between a capture molecule at the test site and a molecule of interest.

2. The system of claim 1, further comprising:

a memory;

a plurality of command instructions stored within the memory; and

a controller operably connected to the memory and to the at least one actuator and configured to execute the plurality of command instructions to control the at least one actuator to actuate the at least one of the at least one activatable stimulant.

3. The system of claim 2, further comprising:

a sensor operably connected to the controller and configured to sense a chemical environmental condition at the at least one test site, wherein the controller is further configured to execute the plurality of command instructions to control the at least one actuator to actuate the at least one of the at least one activatable stimulant based upon the sensed chemical environmental condition at the at least one test site.

4. The system of claim 3, wherein:

the at least one activatable stimulant comprises a first activatable stimulant and a second activatable stimulant;

the first activatable stimulant is configured to activate in response to a first activation input but not a second activation input; and

the second activatable stimulant is configured to activate in response to the second activation input.

5. The system of claim 4, wherein the at least one actuator comprises at least one resonator.

6. The system of claim 3, wherein the at least one actuator comprises at least one electrode.

7. The system of claim 2, wherein the substrate comprises a CMOS substrate.

8. The system of claim 2, wherein the substrate comprises:

a test sample receiving area spaced apart from the at least one test site; and

a fluid path including the test sample receiving area and extending to the at least one test site, wherein the at least one activatable stimulant is positioned on the substrate such that fluid received by the test sample receiving area and transported along the fluid path entrains the at least one activatable stimulant and transports the at least one activatable stimulant to the at least one test site.

9. The system of claim 8, further comprising:

a reader configured to receive at least the portion of the substrate including the at least one test site, the reader including the memory and the controller.

10. The system of claim 1, wherein the substrate is a glass substrate comprising:

at least one electrode etched on the glass substrate; and

a poly-ethyleneglycol (PEG) film.

11. The system of claim 1, wherein the at least one activatable stimulant comprises one or more of an amphoteric electrolyte and a first type of microcapsule bead.

12. The system of claim 11, wherein the at least one activatable stimulant comprises:

the first type of microcapsule bead; and

a second type of microcapsule bead, wherein the second type of microcapsule bead has an activation characteristic different from an activation characteristic of the first type of microcapsule bead.

13. The system of claim 4, wherein:

the first activatable stimulant comprises a first type of microcapsule bead; and

the second activatable stimulant comprises a second type of microcapsule bead.