System and method for a hydrogel and hydrogel composite for cartilage repair applications

• US 9,192,655 B2
• Filed: 03/12/2010
• Issued: 11/24/2015
• Est. Priority Date: 03/12/2009
• Status: Active Grant

• Current Assignee: New Jersey Institute of Technology
• Original Assignee: New Jersey Institute of Technology
• Inventors: Arinzeh, Treena, Collins, George, Mantilla, Bruno
• Primary Examiner(s): Parad, Dennis J
• Application Number:
  US12/661,242
• Publication Number:
  US 20100233234A1
• Time in Patent Office: 2,083 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61K 38/39 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 27/26 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 27/52 Hydrogels or hydrocolloids
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 19/04 for non-specific disorders of the connective tissue
• CPC Class Codes: C08L 1/02 Cellulose; Modified cellulose
• CPC Class Codes: C08L 1/16 Esters of inorganic acids
• CPC Class Codes: D01D 5/0007 Electro-spinning non-woven fabrics produced by electro-spinning D04H1/728
• CPC Class Codes: D01F 2/24 from cellulose derivatives

Abstract

The embodiments and examples provided herein are put forth so as to provide those of ordinary skill in the art with a complete disclosure and description of how to make and use the present invention, and are not intended to limit the present invention relates to compositions and methods of preparing a hydrogel comprising a water soluble cellulose compound, as well as preparing a three-dimensional matrix of micron sized electrospun fibers, wherein the electrospun fibers are formed from a electrospun composite comprising a water soluble cellulose compound. The matrix provides a scaffold supporting and promoting cartilage regeneration and repair.

Claims

1. A fibrous network-hydrogel composition for use in tissue engineering comprising a matrix including a fibrous polymer network formed of at least one of an electrospun degradable polymer, an electrospun nondegradable polymer or a combination thereof, wherein the degradable polymer is poly L-lactic acid (PLLA), wherein the electrospun fibers have an average diameter between about 2 μ

m and about 7 μ

m and are coated with sodium cellulose sulfate (NaCS) wherein the sodium cellulose sulfate is ionically crosslinked onto the fibers, forms a hydrogel, and occupies at least a portion of the interstices between the fibers.

2. The fibrous network-hydrogel composition of claim 1, wherein the fibrous polymer network comprises a nondegradable polymer and a degradable polymer.

3. The fibrous network-hydrogel composition of claim 1, wherein the nondegradable polymer is selected from the group consisting of a polyurethane, a polyvinylidine fluoride, and a polyvinylidine fluoride trifluoroethylene.

4. The fibrous network-hydrogel composition of claim 1, further comprising stem cells.

5. The fibrous network-hydrogel composition of claim 4, wherein the stem cells are mesenchymal stem cells.

6. The fibrous network-hydrogel composition of claim 5, wherein the hydrogel promotes stem cell chrondrogenesis.

7. The fibrous network-hydrogel composition of claim 1, further comprising collagen.

8. The fibrous network-hydrogel composition of claim 1, further comprising a cell growth factor.

9. The fibrous network-hydrogel composition of claim 1, further comprising an unsubstituted soluble polysaccharide.

10. The fibrous network-hydrogel composition of claim 9, wherein the polysaccharide is dextran.

11. The fibrous network-hydrogel composition of claim 1, wherein the fibers have an average diameter of about 5 micrometers.

14. A method of treating or repairing cartilage tissue in a subject in need of such repair, the method comprising administering to said subject an effective amount of a hydrogel according to claim 1.

15. A method of treating arthritis in a subject in need of such treatment, the method comprising administering to said subject an effective amount of a hydrogel according to claim 1.

12. A fibrous network-hydrogel composition formed according to the method comprising:

electrospinning at high voltage a fibrous network formed of at least one of degradable polymer, wherein the degradable polymer is poly L-lactic acid (PLLA), a nondegradable polymer or a combination thereof, wherein the fibers have an average diameter between about 2 μ

m and about 7 μ

m and coating the electrospun fibrous network with sodium cellulose sulfate (NaCS), wherein the sodium cellulose sulfate is ionically crosslinked onto the fibers, forms a hydrogel, and occupies at least a portion of the interstices between the fibers.

13. The fibrous network-hydrogel composition of claim 12, wherein the fibers have an average diameter of about 5 micrometers.