Lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same
First Claim
Patent Images
1. A three-dimensional assay device comprising:
- a plurality of porous, hydrophilic media in the form of substantially planar sheets disposed face-to-face and separated from each other by a fluid-impermeable layer having one or more openings for fluid communication between adjacent sheets,a said sheet comprising a pattern of fluid-impervious barriers substantially inserting through the thickness of the sheet thereby to define a boundary for plural assay regions, andone or more assay reagents disposed in the assay regions which provide an indication in the device of a clinically relevant presence or concentration of an analyte.
1 Assignment
0 Petitions
Accused Products
Abstract
Embodiments of the invention provide lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same. Under one aspect, an assay device includes a porous, hydrophilic medium; a fluid impervious barrier comprising polymerized photoresist, the barrier substantially permeating the thickness of the porous, hydrophilic medium and defining a boundary of an assay region within the porous, hydrophilic medium; and an assay reagent in the assay region.
-
Citations
36 Claims
-
1. A three-dimensional assay device comprising:
-
a plurality of porous, hydrophilic media in the form of substantially planar sheets disposed face-to-face and separated from each other by a fluid-impermeable layer having one or more openings for fluid communication between adjacent sheets, a said sheet comprising a pattern of fluid-impervious barriers substantially inserting through the thickness of the sheet thereby to define a boundary for plural assay regions, and one or more assay reagents disposed in the assay regions which provide an indication in the device of a clinically relevant presence or concentration of an analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
-
Specification