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Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide

  • US 9,194,838 B2
  • Filed: 03/01/2011
  • Issued: 11/24/2015
  • Est. Priority Date: 03/03/2010
  • Status: Active Grant
First Claim
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1. A method for sequencing a nucleic acid molecule, comprising:

  • (a) providing a solid substrate comprising a pair of electrodes that at least partially define a gap, wherein said gap has a distance that is less than a molecular diameter of said nucleic acid molecule;

    (b) moving said nucleic acid molecule through said gap;

    (c) detecting pulses of a tunnel current across said gap using said pair of electrodes, which pulses correspond to individual nucleic acid subunits of said nucleic acid molecule;

    (d) calculating a modal value of current of said pulses detected in (c); and

    (e) comparing said modal value calculated in (d) against a reference value to identify an individual nucleic acid subunit of said nucleic acid molecule.

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